Comparative Survival and the Cold-Induced Gene Expression of Pathogenic and Nonpathogenic Vibrio Parahaemolyticus from Tropical Eastern Oysters during Cold Storage

Francisco Alarcón Elvira, Violeta T. Pardío Sedas, David Martínez Herrera, Rodolfo Quintana Castro, Rosa María Oliart Ros, Karla López Hernández, Argel Flores Primo and Karen Ramírez Elvira
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Francisco Alarcón Elvira: Facultad de Medicina Veterinaria y Zootecnia, Universidad Veracruzana, Av. Miguel Ángel de Quevedo s/n esq. Yáñez, Col. Unidad Veracruzana, Veracruz, Ver. CP 91710, Mexico
Violeta T. Pardío Sedas: Facultad de Medicina Veterinaria y Zootecnia, Universidad Veracruzana, Av. Miguel Ángel de Quevedo s/n esq. Yáñez, Col. Unidad Veracruzana, Veracruz, Ver. CP 91710, Mexico
David Martínez Herrera: Facultad de Medicina Veterinaria y Zootecnia, Universidad Veracruzana, Av. Miguel Ángel de Quevedo s/n esq. Yáñez, Col. Unidad Veracruzana, Veracruz, Ver. CP 91710, Mexico
Rodolfo Quintana Castro: Facultad de Bioanálisis, Universidad Veracruzana, Calle Iturbide s/n, Col. Centro, Veracruz, Ver. CP 91700, Mexico
Rosa María Oliart Ros: Unidad de Investigación y Desarrollo en Alimentos, Instituto Tecnológico de Veracruz, Av. Miguel A. de Quevedo 2779, Veracruz, Ver. 91897, Mexico
Karla López Hernández: Facultad de Medicina Veterinaria y Zootecnia, Universidad Veracruzana, Av. Miguel Ángel de Quevedo s/n esq. Yáñez, Col. Unidad Veracruzana, Veracruz, Ver. CP 91710, Mexico
Argel Flores Primo: Facultad de Medicina Veterinaria y Zootecnia, Universidad Veracruzana, Av. Miguel Ángel de Quevedo s/n esq. Yáñez, Col. Unidad Veracruzana, Veracruz, Ver. CP 91710, Mexico
Karen Ramírez Elvira: Facultad de Medicina Veterinaria y Zootecnia, Universidad Veracruzana, Av. Miguel Ángel de Quevedo s/n esq. Yáñez, Col. Unidad Veracruzana, Veracruz, Ver. CP 91710, Mexico

IJERPH, 2020, vol. 17, issue 6, 1-16

Abstract: Expression of the regulatory stress rpoS gene controls the transcription of cspA genes, which are involved in survival and adaptation to low temperatures. The purpose of this study was to assess the growth kinetics of naturally occurring V . parahaemolyticus in shellstock oysters and in vitro and the cold-shock-induced expression of the rpoS and cspA gene response in vitro during postharvest refrigeration. Naturally contaminated eastern oysters ( Crassostrea virginica ) and pathogenic ( Vp-tdh ) and nonpathogenic ( Vp-tlh ) isolates were stored at 7 ± 1 °C for 168 h and 216 h, respectively. The regulatory stress ( rpos ) and cold-shock ( cspA ) gene expressions were determined by reverse transcription PCR. At 24 h, the ( Vp-tdh ) strain grew faster ( p < 0.05) than the ( Vp-tlh ) strain in oysters (λ = 0.33, 0.39, respectively) and in vitro (λ = 0.89, 37.65, respectively), indicating a better adaptation to cold shock for the ( Vp-tdh ) strain in live oysters and in vitro. At 24 h, the ( Vp - tdh ) strain rpoS and cspA gene expressions were upregulated by 1.9 and 2.3-fold, respectively, but the ( Vp-tlh) strain rpoS and cspA gene expressions were repressed and upregulated by −0.024 and 1.9-fold, respectively. The V . parahaemolyticus strains that were isolated from tropical oysters have adaptive expression changes to survive and grow at 7 °C, according to their virulence.

Keywords: Vibrio parahaemolyticus; postharvest refrigeration; cold shock; growth kinetics; gene expression (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2020
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