Occurrence of KPC-Producing Escherichia coli in Psittaciformes Rescued from Trafficking in Paraíba, Brazil

Gedean Galdino da Cruz Silva, Eloiza Helena Campana, Priscylla Carvalho Vasconcelos, Núbia Michelle Vieira da Silva, Lauro Santos Filho, Elma Lima Leite, Patrícia Emília Naves Givisiez, Wondwossen Abebe Gebreyes and Celso José Bruno de Oliveira
Additional contact information
Gedean Galdino da Cruz Silva: Laboratório de Avaliação de Produtos de Origem Animal (LAPOA), Departamento de Zootecnia, Centro de Ciências Agrárias, Universidade Federal da Paraíba, Areia-PB 58397-000, Brazil
Eloiza Helena Campana: Laboratório de Microbiologia Clínica (LMC), Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal da Paraíba, João Pessoa-PB 58033-455, Brazil
Priscylla Carvalho Vasconcelos: Laboratório de Avaliação de Produtos de Origem Animal (LAPOA), Departamento de Zootecnia, Centro de Ciências Agrárias, Universidade Federal da Paraíba, Areia-PB 58397-000, Brazil
Núbia Michelle Vieira da Silva: Laboratório de Avaliação de Produtos de Origem Animal (LAPOA), Departamento de Zootecnia, Centro de Ciências Agrárias, Universidade Federal da Paraíba, Areia-PB 58397-000, Brazil
Lauro Santos Filho: Laboratório de Microbiologia Clínica (LMC), Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal da Paraíba, João Pessoa-PB 58033-455, Brazil
Elma Lima Leite: Laboratório de Avaliação de Produtos de Origem Animal (LAPOA), Departamento de Zootecnia, Centro de Ciências Agrárias, Universidade Federal da Paraíba, Areia-PB 58397-000, Brazil
Patrícia Emília Naves Givisiez: Laboratório de Avaliação de Produtos de Origem Animal (LAPOA), Departamento de Zootecnia, Centro de Ciências Agrárias, Universidade Federal da Paraíba, Areia-PB 58397-000, Brazil
Wondwossen Abebe Gebreyes: Global One Health Initiative (GOHi), Ohio State University, Columbus, OH 43210, USA
Celso José Bruno de Oliveira: Laboratório de Avaliação de Produtos de Origem Animal (LAPOA), Departamento de Zootecnia, Centro de Ciências Agrárias, Universidade Federal da Paraíba, Areia-PB 58397-000, Brazil

IJERPH, 2020, vol. 18, issue 1, 1-9

Abstract: The emergence and spread of antimicrobial resistance pose a threat to public health globally. Antibiotic-resistant bacteria and genes can disseminate among environments, animals and humans. Therefore, investigation into potential reservoirs of multidrug-resistant bacteria is of great importance to the understanding of putative transmission routes of resistant bacteria and resistance genes. This study aimed to report the occurrence of Escherichia coli harboring the Klebsiella pneumoniae carbapenemase-producing gene ( bla KPC ) in Psittaciformes rescued from wildlife trafficking in Paraíba State, Brazil. Cloacal swabs were collected from thirty birds and cultured by conventional microbiology using MacConkey and serum tryptone glucose glycerol (STGG) media supplemented with selective antimicrobials. E. coli isolates (n = 43) were identified by phenotypic tests and confirmed by MALDI-TOF. Antimicrobial susceptibility profiles were determined by means of Kirby–Bauer test. All isolates were further screened for extended-spectrum beta-lactamase (ESBL) production, and putative genes encoding ESBL were investigated by PCR. Additionally, bla KPC -harboring strains were genotyped by REP-PCR. A total of 43 E. coli phenotypically resistant isolates were recovered. The highest resistance rate was observed against ciprofloxacin. Among the resistance genes, only bla KPC was found in seven different birds from three species. According to the genotyping, these seven isolates belonged to four different strains. To date, this is the first report on the occurrence of KPC- E. coli in Psittaciformes rescued from trafficking in Northeastern Brazil. Due to the high clinical importance of KPC- E. coli , our findings suggest that wild animals in captivity at wildlife rescue centers can play a role as reservoirs of bacteria that are resistance to Critically Important antimicrobials in human medicine.

Keywords: antimicrobials; resistance genes; wildlife (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2020
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