Application of EN 16615 (4-Field Test) for the Evaluation of the Antimicrobial Activity of the Selected Commercial and Self-Made Disinfectant Wipes

Stefan Tyski, Wanda Grzybowska and Ewa Bocian
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Stefan Tyski: Department of Antibiotics and Microbiology, National Medicines Institute, 00-725 Warsaw, Poland
Wanda Grzybowska: Department of Antibiotics and Microbiology, National Medicines Institute, 00-725 Warsaw, Poland
Ewa Bocian: Department of Antibiotics and Microbiology, National Medicines Institute, 00-725 Warsaw, Poland

IJERPH, 2021, vol. 18, issue 11, 1-11

Abstract: The purpose of disinfectants is to reduce microorganisms on a contaminated surface and to prevent the spread of microorganisms. The relatively new EN 16615 simulates disinfection by wiping and allows for assessing the recovery of microorganisms from the surface and, importantly, the degree of spread of microorganisms when the surface is disinfected by wiping. For the first time, using this standard, the tested products in the form of commercial disinfectant wipes were compared with self-made wipes soaked in respective disinfectant liquids. The disinfected surfaces were simulated by homogeneous polyvinyl chloride plates. The studies were carried out not only with the standard, but also with clinical multidrug-resistant microbial strains. Based on the research, it can be concluded that the most effective products in the disinfection process (log 10 reduction of ?5) with the shortest contact time (1 min) were products containing ethanol, propanol, and quaternary ammonium compounds (self-made wipes) and propanol (commercial wipes). The least effective products (log 10 reduction of <5) in terms of the contact time declared by the manufacturer were products containing ethanol and sodium hypochlorite (commercial wipes). Much better antimicrobial activity of self-made wipes was observed in comparison to the activity of the commercial wipes.

Keywords: biocidal efficacy; antimicrobial activity; disinfection; disinfectant agents; EN 16615 (4-field test); commercial disinfectant wipes; self-made wipes (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2021
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