Prenatal Bisphenol a Exposure, DNA Methylation, and Low Birth Weight: A Pilot Study in Taiwan

Yu-Fang Huang, Chia-Huang Chang, Pei-Jung Chen, I-Hsuan Lin, Yen-An Tsai, Chian-Feng Chen, Yu-Chao Wang, Wei-Yun Huang, Ming-Song Tsai and Mei-Lien Chen
Additional contact information
Yu-Fang Huang: Department of Safety, Health and Environmental Engineering, National United University, Miaoli 360, Taiwan
Chia-Huang Chang: School of Public Health, Taipei Medical University, Taipei 110, Taiwan
Pei-Jung Chen: Institute of Environmental and Occupational Health Sciences, School of Medicine, National Yang Ming Chiao Tung University, Taipei 112, Taiwan
I-Hsuan Lin: VYM Genome Research Center, National Yang Ming Chiao Tung University, Taipei 112, Taiwan
Yen-An Tsai: Institute of Environmental and Occupational Health Sciences, School of Medicine, National Yang Ming Chiao Tung University, Taipei 112, Taiwan
Chian-Feng Chen: VYM Genome Research Center, National Yang Ming Chiao Tung University, Taipei 112, Taiwan
Yu-Chao Wang: Institute of Biomedical Informatics, National Yang Ming Chiao Tung University, Taipei 112, Taiwan
Wei-Yun Huang: Immuno Genomics Co., Ltd., Taipei 112, Taiwan
Ming-Song Tsai: Department of Obstetrics and Gynecology, Cathay General Hospital, Taipei 110, Taiwan
Mei-Lien Chen: Institute of Environmental and Occupational Health Sciences, School of Medicine, National Yang Ming Chiao Tung University, Taipei 112, Taiwan

IJERPH, 2021, vol. 18, issue 11, 1-14

Abstract: Prenatal exposure to bisphenol A (BPA) may increase the risk of abnormal birth outcomes, and DNA methylation might mediate these adverse effects. This study aimed to investigate the effects of maternal BPA exposure on maternal and fetal DNA methylation levels and explore whether epigenetic changes are related to the associations between BPA and low birth weight. We collected urine and blood samples originating from 162 mother-infant pairs in a Taiwanese cohort study. We measured DNA methylation using the Illumina Infinium HumanMethylation 450 BeadChip in 34 maternal blood samples with high and low BPA levels based on the 75th percentile level (9.5 ?g/g creatinine). Eighty-seven CpGs with the most differentially methylated probes possibly interacting with BPA exposure or birth weight were selected using two multiple regression models. Ingenuity pathway analysis (IPA) was utilized to narrow down 18 candidate CpGs related to disease categories, including developmental disorders, skeletal and muscular disorders, skeletal and muscular system development, metabolic diseases, and lipid metabolism. We then validated these genes by pyrosequencing, and 8 CpGs met the primer design score requirements in 82 cord blood samples. The associations among low birth weight, BPA exposure, and DNA methylation were analyzed. Exposure to BPA was associated with low birth weight. Analysis of the epigenome-wide findings did not show significant associations between BPA and DNA methylation in cord blood of the 8 CpGs. However, the adjusted odds ratio for the dehydrogenase/reductase member 9 (DHRS9) gene, at the 2nd CG site, in the hypermethylated group was significantly associated with low birth weight. These results support a role of BPA, and possibly DHRS9 methylation, in fetal growth. However, additional studies with larger sample sizes are warranted.

Keywords: bisphenol A; DNA methylation; Illumina HumanMethylation 450 BeadChip; birth outcomes (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2021
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.mdpi.com/1660-4601/18/11/6144/pdf (application/pdf)
https://www.mdpi.com/1660-4601/18/11/6144/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jijerp:v:18:y:2021:i:11:p:6144-:d:570077

Access Statistics for this article

IJERPH is currently edited by Ms. Jenna Liu

More articles in IJERPH from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().