Two-Photon Laser Ablation and In Vivo Wide-Field Imaging of Inferior Olive Neurons Revealed the Recovery of Olivocerebellar Circuits in Zebrafish

Kanae Hiyoshi, Kaito Saito, Narumi Fukuda, Takahisa Matsuzaki, Hiroshi Y. Yoshikawa and Sachiko Tsuda
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Kanae Hiyoshi: Division of Life Science, Graduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan
Kaito Saito: Division of Life Science, Graduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan
Narumi Fukuda: Division of Life Science, Graduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan
Takahisa Matsuzaki: Division of Strategic Research and Development, Graduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan
Hiroshi Y. Yoshikawa: Division of Strategic Research and Development, Graduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan
Sachiko Tsuda: Division of Life Science, Graduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan

IJERPH, 2021, vol. 18, issue 16, 1-11

Abstract: The cerebellum, a brain region with a high degree of plasticity, is pivotal in motor control, learning, and cognition. The cerebellar reserve is the capacity of the cerebellum to respond and adapt to various disorders via resilience and reversibility. Although structural and functional recovery has been reported in mammals and has attracted attention regarding treatments for cerebellar dysfunction, such as spinocerebellar degeneration, the regulatory mechanisms of the cerebellar reserve are largely unidentified, particularly at the circuit level. Herein, we established an optical approach using zebrafish, an ideal vertebrate model in optical techniques, neuroscience, and developmental biology. By combining two-photon laser ablation of the inferior olive (IO) and long-term non-invasive imaging of “the whole brain” at a single-cell resolution, we succeeded in visualization of the morphological changes occurring in the IO neuron population and showed at a single-cell level that structural remodeling of the olivocerebellar circuit occurred in a relatively short period. This system, in combination with various functional analyses, represents a novel and powerful approach for uncovering the mechanisms of the cerebellar reserve, and highlights the potential of the zebrafish model to elucidate the organizing principles of neuronal circuits and their homeostasis in health and disease.

Keywords: two-photon laser ablation; in vivo imaging; zebrafish; cerebellar reserve; olivocerebellar circuit; inferior olive; climbing fiber (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2021
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