Evaluation of an Environmental Transport Medium for Legionella pneumophila Recovery

Marianna Martinelli, Enrico Calaresu, Rosario Musumeci, Chiara Giubbi, Federica Perdoni, Sergio Frugoni, Santina Castriciano, Maria Scaturro, Maria Luisa Ricci and Clementina E. Cocuzza
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Marianna Martinelli: Department of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, Italy
Enrico Calaresu: Department of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, Italy
Rosario Musumeci: Department of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, Italy
Chiara Giubbi: Department of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, Italy
Federica Perdoni: Department of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, Italy
Sergio Frugoni: Department of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, Italy
Santina Castriciano: Copan Italia SpA, 25125 Brescia, Italy
Maria Scaturro: Istituto Superiore di Sanità, 00161 Roma, Italy
Maria Luisa Ricci: Istituto Superiore di Sanità, 00161 Roma, Italy
Clementina E. Cocuzza: Department of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, Italy

IJERPH, 2021, vol. 18, issue 16, 1-8

Abstract: The collection and storage of water-related matrices such as biofilm from collection to processing are critical for the detection of Legionella pneumophila by cultural and molecular tests. SRK™ is a liquid medium that acts both as an antimicrobial neutralizing agent and a transport medium for bacterial culture enumeration and is useful to maintain the stability of the sample from collection to analysis. The aims of this study were to evaluate Legionella pneumophila viability and bacterial nucleic acids’ stability in SRK™ medium over time at different storage conditions. Artificial bacterial inoculates with an approximate concentration of 10 4 , 10 3 and 10 2 CFU/mL were made using Legionella pneumophila certified reference material suspended in SRK™ medium. Bacteria recovery was analyzed by cultural and molecular methods at time 0, 24 and 48 h at room temperature and at 0, 24, 48 and 72 h at 2–8 °C, respectively. SRK™ medium supported Legionella pneumophila culture viability with CFU counts within the expected range. The recovery after 72 h at 2–8 °C was 83–100% and 75–95% after 48 h at room temperature. Real-time PCR appropriately detected Legionella pneumophila DNA at each temperature condition, dilution and time point. Results demonstrated a good performance of SRK™ medium for the reliable recovery of environmental Legionella .

Keywords: Legionella pneumophila; culture method; molecular detection; transport media; ISO 11731:2017 (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2021
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