Design of a Microbial Remediation Inoculation Program for Petroleum Hydrocarbon Contaminated Sites Based on Degradation Pathways

Xingchun Li, Wei He, Meijin Du, Jin Zheng, Xianyuan Du and Yu Li
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Xingchun Li: State Key Laboratory of Petroleum Pollution Control, Beijing 102206, China
Wei He: MOE Key Laboratory of Resources Environmental Systems Optimization, North China Electric Power University, Beijing 102206, China
Meijin Du: MOE Key Laboratory of Resources Environmental Systems Optimization, North China Electric Power University, Beijing 102206, China
Jin Zheng: State Key Laboratory of Petroleum Pollution Control, Beijing 102206, China
Xianyuan Du: State Key Laboratory of Petroleum Pollution Control, Beijing 102206, China
Yu Li: MOE Key Laboratory of Resources Environmental Systems Optimization, North China Electric Power University, Beijing 102206, China

IJERPH, 2021, vol. 18, issue 16, 1-18

Abstract: This paper analyzed the degradation pathways of petroleum hydrocarbon degradation bacteria, screened the main degradation pathways, and found the petroleum hydrocarbon degradation enzymes corresponding to each step of the degradation pathway. Through the Copeland method, the best inoculation program of petroleum hydrocarbon degradation bacteria in a polluted site was selected as follows: single oxygenation path was dominated by Streptomyces avermitilis , hydroxylation path was dominated by Methylosinus trichosporium OB3b, secondary oxygenation path was dominated by Pseudomonas aeruginosa , secondary hydroxylation path was dominated by Methylococcus capsulatus , double oxygenation path was dominated by Acinetobacter baylyi ADP1, hydrolysis path was dominated by Rhodococcus erythropolis , and CoA path was dominated by Geobacter metallireducens GS-15 to repair petroleum hydrocarbon contaminated sites. The Copeland method score for this solution is 22, which is the highest among the 375 solutions designed in this paper, indicating that it has the best degradation effect. Meanwhile, we verified its effect by the Cdocker method, and the Cdocker energy of this solution is ?285.811 kcal/mol, which has the highest absolute value. Among the inoculation programs of the top 13 petroleum hydrocarbon degradation bacteria, the effect of the best inoculation program of petroleum hydrocarbon degradation bacteria was 18% higher than that of the 13th group, verifying that this solution has the best overall degradation effect. The inoculation program of petroleum hydrocarbon degradation bacteria designed in this paper considered the main pathways of petroleum hydrocarbon pollutant degradation, especially highlighting the degradability of petroleum hydrocarbon intermediate degradation products, and enriching the theoretical program of microbial remediation of petroleum hydrocarbon contaminated sites.

Keywords: petroleum hydrocarbons; remediation of petroleum contaminated sites; microbial remediation; molecular dynamics modelling; Copeland method (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2021
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