Salmonella enterica Serovar Enteritidis Control in Poultry Litter Mediated by Lytic Bacteriophage Isolated from Swine Manure

Paula Rogovski, Raphael da Silva, Rafael Dorighello Cadamuro, Estêvão Brasiliense de Souza, Beatriz Pereira Savi, Aline Viancelli, William Michelon, Deisi Cristina Tápparo, Helen Treichel, David Rodríguez-Lazaro and Gislaine Fongaro
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Paula Rogovski: Laboratory of Applied Virology, Department of Microbiology, Immunology and Parasitology, Federal University of Santa Catarina, Florianópolis 88040-900, SC, Brazil
Raphael da Silva: Laboratory of Applied Virology, Department of Microbiology, Immunology and Parasitology, Federal University of Santa Catarina, Florianópolis 88040-900, SC, Brazil
Rafael Dorighello Cadamuro: Laboratory of Applied Virology, Department of Microbiology, Immunology and Parasitology, Federal University of Santa Catarina, Florianópolis 88040-900, SC, Brazil
Estêvão Brasiliense de Souza: Laboratory of Applied Virology, Department of Microbiology, Immunology and Parasitology, Federal University of Santa Catarina, Florianópolis 88040-900, SC, Brazil
Beatriz Pereira Savi: Laboratory of Applied Virology, Department of Microbiology, Immunology and Parasitology, Federal University of Santa Catarina, Florianópolis 88040-900, SC, Brazil
Aline Viancelli: Research Group on Engineering, Performance and Environmental Quality, Universidade do Contestado (PMPECSA), Concórdia 89711-330, SC, Brazil
William Michelon: Research Group on Engineering, Performance and Environmental Quality, Universidade do Contestado (PMPECSA), Concórdia 89711-330, SC, Brazil
Deisi Cristina Tápparo: Centro de Engenharias e Ciências Exatas, Western Paraná State University, Cascavel 85819-110, PR, Brazil
Helen Treichel: Laboratory of Microbiology and Bioprocesses, Federal University of Fronteira Sul, Erechim 89802-112, RS, Brazil
David Rodríguez-Lazaro: Division of Microbiology, Department of Biotechnology and Food Science, University of Burgos, 09001 Burgos, Spain
Gislaine Fongaro: Laboratory of Applied Virology, Department of Microbiology, Immunology and Parasitology, Federal University of Santa Catarina, Florianópolis 88040-900, SC, Brazil

IJERPH, 2021, vol. 18, issue 16, 1-9

Abstract: We report the use of bacteriophages for control of Salmonella Enteritidis in poultry production. Phage was isolated by the double-agar plate assay from agricultural waste samples, and one isolate, named SM1, was selected and propagated for application in poultry litter. Two experimental protocols were tested: single treatment and repeated treatment (re-application of phage SM1 after 6 h and 12 h). Each treatment cycle involved 25 g of poultry litter placed in plastic boxes and contaminated with 10 5 Colony Forming Units mL ?1 (CFU mL ?1 ) of S. Enteritidis, in independent duplicates. The contaminated litter was treated with 10 6 Plaque Forming Units mL ?1 (PFU mL ?1 ) of SM1 phage by dripping. Repeated application of phage SM1 reduced Salmonella counts by over 99.9%; the phage persisted in poultry litter for over 35 days. This study illustrates the application of SM1 treatment as a promising technology for bacterial control in production matrices that could allow safe and sustainable use of agricultural waste products as biofertilizers.

Keywords: environmental management; food safety; bacteriophage isolation; Salmonella; foodborne pathogen control (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2021
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