Towards A Novel Multi-Epitopes Chimeric Vaccine for Simulating Strong Immune Responses and Protection against Morganella morganii

Asad Ullah, Sajjad Ahmad, Saba Ismail, Zobia Afsheen, Muhammad Khurram, Muhammad Tahir ul Qamar, Naif AlSuhaymi, Mahdi H. Alsugoor and Khaled S. Allemailem
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Asad Ullah: Department of Health and Biological Sciences, Abasyn University, Peshawar 25000, Pakistan
Sajjad Ahmad: Department of Health and Biological Sciences, Abasyn University, Peshawar 25000, Pakistan
Saba Ismail: Department of Biological Sciences, National University of Medical Sciences, Rawalpindi 46000, Pakistan
Zobia Afsheen: Department of Health and Biological Sciences, Abasyn University, Peshawar 25000, Pakistan
Muhammad Khurram: Department of Health and Biological Sciences, Abasyn University, Peshawar 25000, Pakistan
Muhammad Tahir ul Qamar: College of Life Science and Technology, Guangxi University, Nanning 530004, China
Naif AlSuhaymi: Department of Emergency Medical Services, Faculty of Health Sciences, AlQunfudah, Umm Al-Qura University, Makkah 21912, Saudi Arabia
Mahdi H. Alsugoor: Department of Emergency Medical Services, Faculty of Health Sciences, AlQunfudah, Umm Al-Qura University, Makkah 21912, Saudi Arabia
Khaled S. Allemailem: Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Buraydah 51452, Saudi Arabia

IJERPH, 2021, vol. 18, issue 20, 1-26

Abstract: Morganella morganii is one of the main etiological agents of hospital-acquired infections and no licensed vaccine is available against the pathogen. Herein, we designed a multi-epitope-based vaccine against M. morganii . Predicted proteins from fully sequenced genomes of the pathogen were subjected to a core sequences analysis, followed by the prioritization of non-redundant, host non-homologous and extracellular, outer membrane and periplasmic membrane virulent proteins as vaccine targets. Five proteins (TonB-dependent siderophore receptor, serralysin family metalloprotease, type 1 fimbrial protein, flagellar hook protein (FlgE), and pilus periplasmic chaperone) were shortlisted for the epitope prediction. The predicted epitopes were checked for antigenicity, toxicity, solubility, and binding affinity with the DRB*0101 allele. The selected epitopes were linked with each other through GPGPG linkers and were joined with the cholera toxin B subunit (CTBS) to boost immune responses. The tertiary structure of the vaccine was modeled and blindly docked with MHC-I, MHC-II, and Toll-like receptors 4 (TLR4). Molecular dynamic simulations of 250 nanoseconds affirmed that the designed vaccine showed stable conformation with the receptors. Further, intermolecular binding free energies demonstrated the domination of both the van der Waals and electrostatic energies. Overall, the results of the current study might help experimentalists to develop a novel vaccine against M. morganii .

Keywords: Morganella morganii; multi-epitopes vaccine; pan-genomics; reverse vaccinology; molecular dynamics simulations; binding free energies (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2021
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