Electrophoretic Determination of Trimethylamine (TMA) in Biological Samples as a Novel Potential Biomarker of Cardiovascular Diseases Methodological Approach

Marek Konop, Mateusz Rybka, Emilia Waraksa, Anna K. Laskowska, Artur Nowiński, Tomasz Grzywacz, Wojciech J. Karwowski, Adrian Drapała and Ewa Maria Kłodzińska
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Marek Konop: Department of Experimental Physiology and Pathophysiology, Laboratory of Centre for Preclinical Research, Medical University of Warsaw, 02-106 Warsaw, Poland
Mateusz Rybka: Department of Experimental Physiology and Pathophysiology, Laboratory of Centre for Preclinical Research, Medical University of Warsaw, 02-106 Warsaw, Poland
Emilia Waraksa: Department of Analytical Chemistry and Instrumental Analysis, Institute of Sport—National Research Institute, 01-879 Warsaw, Poland
Anna K. Laskowska: Department of Pharmaceutical Microbiology, Centre for Preclinical Research and Technology (CePT), Faculty of Pharmacy, Medical University of Warsaw, Banacha 1B, 02-097 Warsaw, Poland
Artur Nowiński: Department of Experimental Physiology and Pathophysiology, Laboratory of Centre for Preclinical Research, Medical University of Warsaw, 02-106 Warsaw, Poland
Tomasz Grzywacz: Department of Sport, Institute of Physical Culture, Kazimierz Wielki University, 85-064 Bydgoszcz, Poland
Wojciech J. Karwowski: Department of Measurement and Electronics, Faculty of Electrical Engineering, Automatics, Computer Science and Biomedical Engineering, AGH University of Science and Technology, 02-106 Kraków, Poland
Adrian Drapała: Department of Experimental Physiology and Pathophysiology, Laboratory of Centre for Preclinical Research, Medical University of Warsaw, 02-106 Warsaw, Poland
Ewa Maria Kłodzińska: Department of Analytical Chemistry and Instrumental Analysis, Institute of Sport—National Research Institute, 01-879 Warsaw, Poland

IJERPH, 2021, vol. 18, issue 23, 1-11

Abstract: In competitive athletes, the differential diagnosis between nonpathological changes in cardiac morphology associated with training (commonly referred to as “athlete’s heart”) and certain cardiac diseases with the potential for sudden death is an important and not uncommon clinical problem. The use of noninvasive, fast, and cheap analytical techniques can help in making diagnostic differentiation and planning subsequent clinical strategies. Recent studies have demonstrated the role of gut microbiota and their metabolites in the onset and the development of cardiovascular diseases. Trimethylamine (TMA), a gut bacteria metabolite consisting of carnitine and choline, has recently emerged as a potentially toxic molecule to the circulatory system. The present work aims to develop a simple and cost-effective capillary electrophoresis-based method for the determination of TMA in biological samples. Analytical characteristics of the proposed method were evaluated through the study of its linearity (R 2 > 0.9950) and the limit of detection and quantification (LOD = 1.2 µg/mL; LOQ = 3.6 µg/mL). The method shows great potential in high-throughput screening applications for TMA analysis in biological samples as a novel potential biomarker of cardiovascular diseases. The proposed electrophoretic method for the determination of TMA in biological samples from patients with cardiac disease is now in progress.

Keywords: cardiovascular diseases in athletes; biomarkers; determination of trimethylamine (TMA); capillary electrophoresis (CE) (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2021
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