Bioremediation of Petroleum Hydrocarbons Using Acinetobacter sp. SCYY-5 Isolated from Contaminated Oil Sludge: Strategy and Effectiveness Study

Yiyun Cai, Runkai Wang, Pinhua Rao, Baichun Wu, Lili Yan, Lijiang Hu, Sangsook Park, Moonhee Ryu and Xiaoya Zhou
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Yiyun Cai: School of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai 201620, China
Runkai Wang: School of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai 201620, China
Pinhua Rao: School of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai 201620, China
Baichun Wu: State Key Laboratory of Petroleum Pollution Control, Beijing 102206, China
Lili Yan: School of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai 201620, China
Lijiang Hu: School of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai 201620, China
Sangsook Park: Department of Environmental Engineering, Sunchon National University, 255 Jungang-ro, Suncheon, Jeonnam 57922, Korea
Moonhee Ryu: Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570-752, Korea
Xiaoya Zhou: School of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai 201620, China

IJERPH, 2021, vol. 18, issue 2, 1-14

Abstract: Biodegradation has been considered as an ideal technique for total petroleum hydrocarbon (TPH) contamination, but its efficiency is limited by its application in the field. Herein, an original TPH-degrading strain, SCYY-5, was isolated from contaminated oil sludge and identified as Acinetobacter sp. by 16S rDNA sequence analysis. The biological function of the isolate was investigated by heavy metal tolerance, carbon, and nitrogen source and degradation tests. To enhance its biodegradation efficiency, the response surface methodology (RSM) based on a function model was adopted to investigate and optimize the strategy of microbial and environmental variables for TPH removal. Furthermore, the performance of the system increased to 79.94% with the further addition of extra nutrients, suggesting that the RSM and added nutrients increased the activity of bacteria to meet the needs of the co-metabolism matrix during growth or degradation. These results verified that it is feasible to adopt the optimal strategy of combining bioremediation with RSM to improve the biodegradation efficiency, for contaminated oil sludge.

Keywords: biodegradation; bioremediation; TPH; Acinetobacter sp.; 16S rDNA; response surface methodology (RSM) (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2021
References: View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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