Analysis of Single Nucleotide Variants (SNVs) Induced by Exposure to PM 10 in Lung Epithelial Cells Using Whole Genome Sequencing

Se Jin Park, Gwan Woo Ku, Su Yel Lee, Daeun Kang, Wan Jin Hwang, In Beom Jeong, Sun Jung Kwon, Jaeku Kang and Ji Woong Son
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Se Jin Park: Department of Internal Medicine, Konyang University Hospital, Daejeon 35365, Korea
Gwan Woo Ku: Department of Cardiothoracic Surgery, Konyang University Hospital, Daejeon 35365, Korea
Su Yel Lee: Myunggok Research Institute for Medical Science, Konyang University, Daejeon 35365, Korea
Daeun Kang: Department of Internal Medicine, Konyang University Hospital, Daejeon 35365, Korea
Wan Jin Hwang: Department of Thoracic and Cardiovascular Surgery, Seoul National University Bundang Hospital, Bundang 13620, Korea
In Beom Jeong: Department of Internal Medicine, Konyang University Hospital, Daejeon 35365, Korea
Sun Jung Kwon: Department of Internal Medicine, Konyang University Hospital, Daejeon 35365, Korea
Jaeku Kang: Department of Pharmacology, Myunggok Research Institute, College of Medicine, Konyang University, Daejeon 35365, Korea
Ji Woong Son: Department of Internal Medicine, Konyang University Hospital, Daejeon 35365, Korea

IJERPH, 2021, vol. 18, issue 3, 1-11

Abstract: There are many epidemiological studies asserting that fine dust causes lung cancer, but the biological mechanism is not clear. This study was conducted to investigate the effect of PM 10 (particulate matter less than 10 μm) on single nucleotide variants through whole genome sequencing in lung epithelial cancer cell lines (HCC-827, NCI-H358) that have been exposed to PM 10 . The two cell lines were exposed to PM 10 for 15 days. We performed experimental and next generation sequencing analyses on experimental group that had been exposed to PM 10 as well as an unexposed control group. After exposure to PM 10 , 3005 single nucleotide variants were newly identified in the NCI-H358 group, and 4402 mutations were identified in the HCC-827 group. We analyzed these single nucleotide variants with the Mutalisk program. We observed kataegis in chromosome 1 in NCI-H358 and chromosome 7 in HCC-827. In mutational signatures analysis, the COSMIC mutational signature 5 was highest in both HCC-827 and NCI-H358 groups, and each cosine similarity was 0.964 in HCC-827 and 0.979 in the NCI-H358 group. The etiology of COSMIC mutational signature 5 is unknown at present. Well-designed studies are needed to determine whether environmental factors, such as PM 10 , cause COSMIC mutational signature 5.

Keywords: particulate matter; lung cancer; single nucleotide variants (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2021
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