First Investigative Study of Azole-Resistant Aspergillus fumigatus in the Environment in Burkina Faso

Isidore W. Yerbanga, Agustin Resendiz-Sharpe, Sanata Bamba, Katrien Lagrou, Seydou Nakanabo Diallo, Hector Rodriguez-Villalobos, Olivier Denis and Isabel Montesinos
Additional contact information
Isidore W. Yerbanga: Ecoles Doctorales des Sciences de la Santé, Université Nazi Boni, Bobo-Dioulasso, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso
Agustin Resendiz-Sharpe: Department of Microbiology, Immunology and Transplantation, KU Leuven, University Hospitals Leuven, Leuven, Herestraat 49-Box 1030, 3000 Leuven, Belgium
Sanata Bamba: Ecoles Doctorales des Sciences de la Santé, Université Nazi Boni, Bobo-Dioulasso, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso
Katrien Lagrou: Department of Microbiology, Immunology and Transplantation, KU Leuven, University Hospitals Leuven, Leuven, Herestraat 49-Box 1030, 3000 Leuven, Belgium
Seydou Nakanabo Diallo: Ecoles Doctorales des Sciences de la Santé, Université Nazi Boni, Bobo-Dioulasso, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso
Hector Rodriguez-Villalobos: Department of Microbiology, Cliniques Universitaires Saint-Luc–Université Catholique de Louvain, Brussels, Avenue Hippocrate 10, 1200 Bruxelles, Belgium
Olivier Denis: Department of Microbiology, CHU Namur Site-Godinne, Université Catholique de Louvain, Brussels, Rue Dr Gaston Therasse 1, 5530 Yvoir, Belgium
Isabel Montesinos: Department of Microbiology, LHUB-ULB, Université Libre de Bruxelles, Brussels, Rue Haute 322, 1000 Bruxelles, Belgium

IJERPH, 2021, vol. 18, issue 5, 1-6

Abstract: Azole-resistant Aspergillus fumigatus (ARAF) strains have been reported on all continents, however, limited data exist on these strains in Africa, while several factors, mainly environmental ones, suggest their presence on this continent. This study aimed to assess the environmental prevalence of ARAF strains in Burkina Faso, a country situated in the West African region where data on ARAF is non-existent. In total, 120 environmental samples (soil) were collected and analyzed. Samples were screened for resistance using three azole-containing agar plates; one without azole antifungal (growth control) and two supplemented with either itraconazole (4 mg/L) or voriconazole (2 mg/L). The EUCAST susceptibility testing method was used to confirm the azole-resistant phenotype of A. fumigatus sensu-stricto isolates. Mutations in the cyp51A gene were determined by sequencing. Of the 120 samples, 51 positive samples showed growth of A. fumigatus isolates on control medium. One ARAF (2%; 1/51) isolate was found amongst A. fumigatus positive samples and harbored the F46Y/M172V/E427K cyp51A mutations. No TR34/L98H or TR46/Y121F/T289A mutations were observed. Our study described the first A. fumigatus isolate resistant to an azole antifungal in Burkina Faso.

Keywords: azole-resistance; Aspergillus fumigatus; cyp51A gene; Burkina Faso (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2021
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.mdpi.com/1660-4601/18/5/2250/pdf (application/pdf)
https://www.mdpi.com/1660-4601/18/5/2250/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jijerp:v:18:y:2021:i:5:p:2250-:d:505302

Access Statistics for this article

IJERPH is currently edited by Ms. Jenna Liu

More articles in IJERPH from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().