Improvement of Enzymatic Glucose Conversion from Chestnut Shells through Optimization of KOH Pretreatment

Kang Hyun Lee, Soo Kweon Lee, Jeongho Lee, Seunghee Kim, Chulhwan Park, Seung Wook Kim and Hah Young Yoo
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Kang Hyun Lee: Department of Biotechnology, Sangmyung University, 20, Hongjimun, 2-Gil, Jongno-Gu, Seoul 03016, Korea
Soo Kweon Lee: Department of Chemical and Biological Engineering, Korea University, 145 Anam-Ro, Seongbuk-Gu, Seoul 02841, Korea
Jeongho Lee: Department of Biotechnology, Sangmyung University, 20, Hongjimun, 2-Gil, Jongno-Gu, Seoul 03016, Korea
Seunghee Kim: Department of Biotechnology, Sangmyung University, 20, Hongjimun, 2-Gil, Jongno-Gu, Seoul 03016, Korea
Chulhwan Park: Department of Chemical Engineering, Kwangwoon University, 20 Kwangwoon-ro, Nowon-gu, Seoul 01897, Korea
Seung Wook Kim: Department of Chemical and Biological Engineering, Korea University, 145 Anam-Ro, Seongbuk-Gu, Seoul 02841, Korea
Hah Young Yoo: Department of Biotechnology, Sangmyung University, 20, Hongjimun, 2-Gil, Jongno-Gu, Seoul 03016, Korea

IJERPH, 2021, vol. 18, issue 7, 1-13

Abstract: Worldwide, about one-third of food produced for human consumption is wasted, which includes byproducts from food processing, with a significant portion of the waste still being landfilled. The aim of this study is to convert chestnut shells (CNSs) from food processing into a valuable resource through bioprocesses. Currently, one of the highest barriers to bioprocess commercialization is low conversion of sugar from biomass, and KOH pretreatment was suggested to improve enzymatic digestibility (ED) of CNS. KOH concentration of 3% ( w / w ) was determined as a suitable pretreatment solution by a fundamental experiment. The reaction factors including temperature, time and solid/liquid (S/L) ratio were optimized (77.1 g/L CNS loading at 75 °C for 2.8 h) by response surface methodology (RSM). In the statistical model, temperature and time showed a relatively significant effect on the glucan content (GC) and ED, but S/L ratio was not. GC and ED of the untreated CNS were 45.1% and 12.7%, respectively. On the other hand, GC and ED of pretreated CNS were 83.2% and 48.4%, respectively, and which were significantly improved by about 1.8-fold and 3.8-fold compared to the control group. The improved ED through the optimization is expected to contribute to increasing the value of byproducts generated in food processing.

Keywords: chestnut shells; statistical optimization; KOH pretreatment; enzymatic hydrolysis; food processing wastes (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2021
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