The Acute Effect of Diesel Exhaust Particles and Different Fractions Exposure on Blood Coagulation Function in Mice

Jian Lei, Zhouzhou Li, Xingke Huang, Xin Li, Guangzheng Zhang, Haidong Kan, Renjie Chen and Yuhao Zhang
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Jian Lei: Key Lab of Public Health Safety of the Ministry of Education and NHC Key Laboratory of Health Technology Assessment, School of Public Health, Fudan University, Shanghai 200032, China
Zhouzhou Li: Key Lab of Public Health Safety of the Ministry of Education and NHC Key Laboratory of Health Technology Assessment, School of Public Health, Fudan University, Shanghai 200032, China
Xingke Huang: Key Lab of Public Health Safety of the Ministry of Education and NHC Key Laboratory of Health Technology Assessment, School of Public Health, Fudan University, Shanghai 200032, China
Xin Li: Department of Neurology, Zhongshan Hospital, Fudan University, Shanghai 200032, China
Guangzheng Zhang: Department of Neurology, Zhongshan Hospital, Fudan University, Shanghai 200032, China
Haidong Kan: Key Lab of Public Health Safety of the Ministry of Education and NHC Key Laboratory of Health Technology Assessment, School of Public Health, Fudan University, Shanghai 200032, China
Renjie Chen: Key Lab of Public Health Safety of the Ministry of Education and NHC Key Laboratory of Health Technology Assessment, School of Public Health, Fudan University, Shanghai 200032, China
Yuhao Zhang: Department of Neurology, Zhongshan Hospital, Fudan University, Shanghai 200032, China

IJERPH, 2021, vol. 18, issue 8, 1-9

Abstract: The toxicity and widespread exposure opportunity of diesel exhaust particles (DEP) has aroused public health concerns. This study aimed to investigate the acute effect of DEP and different fractions exposure on blood coagulation function in mice. In this study, nine- week-old C57BL/6J male mice were divided into four exposure groups (with 15 mice in each group). The water-soluble (WS) and water-insoluble (WIS) fractions of DEP were isolated, and intratracheal instillation was used for DEP, WS and WIS exposure. The phosphate buffer saline (PBS) exposure group was set as the control group. After 24 h exposure, the mice were sacrificed for blood routine, coagulation function and bleeding time examinations to estimate the acute effect of DEP, WS and WIS exposure on the blood coagulation function. In our results, no statistically significant difference in weight of body, brain and lung was observed in different exposure groups. While several core indexes in blood coagulation like bleeding time (BT), fibrinogen (FIB), activated partial thromboplastin time (APTT) and prothrombin time (PT) altered or showed a lower tendency after DEP, WS and WIS exposure. For example, BT was lower In WIS exposure group (211.00 s) compared with PBS exposure group (238.50 s) ( p < 0.01), and FIB was lower in WS exposure group (233.00 g/L) compared with PBS exposure group (249.50 g/L) ( p < 0.05). Additionally, systemic inflammation-related indexes like white blood cell count (WBC), neutrophil count (NEUT), lymphocyte count (LYMPH) altered after DEP, WS and WIS exposure. In conclusion, DEP, WS and WIS fractions exposure could result in the hypercoagulable state of blood in mice. The noteworthy effects of WS and WIS fractions exposure on blood coagulation function deserve further investigation of the potential mechanism.

Keywords: diesel exhaust particles; air pollution; blood coagulation; bleeding time; prothrombin (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2021
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