Detection of Dysbiosis and Increased Intestinal Permeability in Brazilian Patients with Relapsing–Remitting Multiple Sclerosis

Felipe Papa Pellizoni, Aline Zazeri Leite, Nathália de Campos Rodrigues, Marcelo Jordão Ubaiz, Marina Ignácio Gonzaga, Nauyta Naomi Campos Takaoka, Vânia Sammartino Mariano, Wellington Pine Omori, Daniel Guariz Pinheiro, Euclides Matheucci Junior, Eleni Gomes and Gislane Lelis Vilela de Oliveira
Additional contact information
Felipe Papa Pellizoni: Microbiome Study Group, School of Health Sciences Dr. Paulo Prata, Barretos 14785-002, Brazil
Aline Zazeri Leite: Microbiology Program, Institute of Biosciences, Humanities and Exact Sciences, São Paulo State University, Sao Jose do Rio Preto 15054-000, Brazil
Nathália de Campos Rodrigues: DNA Consult Genetics and Biotechnology, Sao Carlos 13560-340, Brazil
Marcelo Jordão Ubaiz: Microbiome Study Group, School of Health Sciences Dr. Paulo Prata, Barretos 14785-002, Brazil
Marina Ignácio Gonzaga: Microbiome Study Group, School of Health Sciences Dr. Paulo Prata, Barretos 14785-002, Brazil
Nauyta Naomi Campos Takaoka: Microbiome Study Group, School of Health Sciences Dr. Paulo Prata, Barretos 14785-002, Brazil
Vânia Sammartino Mariano: Barretos Cancer Hospital, Barretos 14784-400, Brazil
Wellington Pine Omori: Department of Technology, School of Agricultural and Veterinarian Sciences, São Paulo State University (UNESP), Jaboticabal 14884-900, Brazil
Daniel Guariz Pinheiro: Department of Technology, School of Agricultural and Veterinarian Sciences, São Paulo State University (UNESP), Jaboticabal 14884-900, Brazil
Euclides Matheucci Junior: Biotechnology Department, Sao Carlos Federal University, Sao Carlos 13565-905, Brazil
Eleni Gomes: Microbiology Program, Institute of Biosciences, Humanities and Exact Sciences, São Paulo State University, Sao Jose do Rio Preto 15054-000, Brazil
Gislane Lelis Vilela de Oliveira: Microbiology Program, Institute of Biosciences, Humanities and Exact Sciences, São Paulo State University, Sao Jose do Rio Preto 15054-000, Brazil

IJERPH, 2021, vol. 18, issue 9, 1-17

Abstract: Dysbiosis, associated with barrier disruption and altered gut–brain communications, has been associated with multiple sclerosis (MS). In this study, we evaluated the gut microbiota in relapsing–remitting patients (RRMS) receiving disease-modifying therapies (DMTs) and correlated these data with diet, cytokines levels, and zonulin concentrations. Stool samples were used for 16S sequencing and real-time PCR. Serum was used for cytokine determination by flow cytometry, and zonulin quantification by ELISA. Pearson’s chi-square, Mann–Whitney, and Spearman’s correlation were used for statistical analyses. We detected differences in dietary habits, as well as in the gut microbiota in RRMS patients, with predominance of Akkermansia muciniphila and Bacteroides vulgatus and decreased Bifidobacterium . Interleukin-6 concentrations were decreased in treated patients, and we detected an increased intestinal permeability in RRMS patients when compared with controls. We conclude that diet plays an important role in the composition of the gut microbiota, and intestinal dysbiosis, detected in RRMS patients could be involved in increased intestinal permeability and affect the clinical response to DTMs. The future goal is to predict therapeutic responses based on individual microbiome analyses (personalized medicine) and propose dietary interventions and the use of probiotics or other microbiota modulators as adjuvant therapy to enhance the therapeutic efficacy of DMTs.

Keywords: autoimmunity; multiple sclerosis; gut microbiota; dysbiosis; inflammation; cytokines; intestinal permeability; disease modifying drugs (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2021
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (2)

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