Occupational Exposure in Industrial Painters: Sensitive and Noninvasive Biomarkers to Evaluate Early Cytotoxicity, Genotoxicity and Oxidative Stress

Delia Cavallo, Cinzia Lucia Ursini, Anna Maria Fresegna, Aureliano Ciervo, Raffaele Maiello, Giuliana Buresti, Enrico Paci, Daniela Pigini, Monica Gherardi, Damiano Carbonari, Renata Sisto, Giovanna Tranfo and Sergio Iavicoli
Additional contact information
Delia Cavallo: Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers’ Compensation Authority—INAIL, Monte Porzio Catone, 00078 Rome, Italy
Cinzia Lucia Ursini: Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers’ Compensation Authority—INAIL, Monte Porzio Catone, 00078 Rome, Italy
Anna Maria Fresegna: Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers’ Compensation Authority—INAIL, Monte Porzio Catone, 00078 Rome, Italy
Aureliano Ciervo: Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers’ Compensation Authority—INAIL, Monte Porzio Catone, 00078 Rome, Italy
Raffaele Maiello: Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers’ Compensation Authority—INAIL, Monte Porzio Catone, 00078 Rome, Italy
Giuliana Buresti: Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers’ Compensation Authority—INAIL, Monte Porzio Catone, 00078 Rome, Italy
Enrico Paci: Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers’ Compensation Authority—INAIL, Monte Porzio Catone, 00078 Rome, Italy
Daniela Pigini: Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers’ Compensation Authority—INAIL, Monte Porzio Catone, 00078 Rome, Italy
Monica Gherardi: Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers’ Compensation Authority—INAIL, Monte Porzio Catone, 00078 Rome, Italy
Damiano Carbonari: Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers’ Compensation Authority—INAIL, Monte Porzio Catone, 00078 Rome, Italy
Renata Sisto: Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers’ Compensation Authority—INAIL, Monte Porzio Catone, 00078 Rome, Italy
Giovanna Tranfo: Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers’ Compensation Authority—INAIL, Monte Porzio Catone, 00078 Rome, Italy
Sergio Iavicoli: Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers’ Compensation Authority—INAIL, Monte Porzio Catone, 00078 Rome, Italy

IJERPH, 2021, vol. 18, issue 9, 1-14

Abstract: This study aimed to identify sensitive and noninvasive biomarkers of early cyto-genotoxic, oxidative and inflammatory effects for exposure to volatile organic compounds (VOCs) in shipyard painters. On 17 (11 spray and 6 roller) painters (previously characterized for VOCs exposure to toluene, xylenes, ethylbenzene, ethyl acetate) and on 18 controls, we performed buccal micronucleus cytome (BMCyt) assay; Fpg-comet assay on lymphocytes; detection of urinary 8-oxoGua (8-oxo-7,8-dihydroguanine), 8-oxodGuo (8-oxo-7,8-dihydro-2?-deoxyguanosine) and 8-oxoGuo (8-oxo-7,8-dihydroguanosine), and cytokines release on serum. We found induction of cyto-genotoxicity by BMCyt assay and inflammatory effects (IL-6 and TNF?) in roller painters exposed to lower VOC concentrations than spray painters. In contrast, in both worker groups, we found direct and oxidative DNA damage by comet assay (with slightly higher oxidative DNA damage in roller) and significant increase of 8-oxoGuo and decrease of 8-oxodGuo and 8-oxoGua in respect to controls. The cyto-genotoxicity observed only on buccal cells of roller painters could be related to the task’s specificity and the different used protective equipment. Although limited by the small number of subjects, the study shows the usefulness of all the used biomarkers in the risk assessment of painters workers exposed to complex mixtures.

Keywords: occupational VOCs exposure; painters; biological monitoring; BMCyt assay; Fpg comet assay; oxidative stress biomarkers; proinflammatory effects (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2021
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