The First Polish Isolate of a Novel Species Pectobacterium aquaticum Originates from a Pomeranian Lake

Weronika Babinska, Agata Motyka-Pomagruk, Wojciech Sledz, Agnieszka Kowalczyk, Zbigniew Kaczynski and Ewa Lojkowska
Additional contact information
Weronika Babinska: Laboratory of Plant Protection and Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk, 58 Abrahama, 80-307 Gdansk, Poland
Agata Motyka-Pomagruk: Laboratory of Plant Protection and Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk, 58 Abrahama, 80-307 Gdansk, Poland
Wojciech Sledz: Laboratory of Plant Protection and Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk, 58 Abrahama, 80-307 Gdansk, Poland
Agnieszka Kowalczyk: Laboratory of Structural Biochemistry, Faculty of Chemistry, University of Gdansk, 63 Wita Stwosza, 80-308 Gdansk, Poland
Zbigniew Kaczynski: Laboratory of Structural Biochemistry, Faculty of Chemistry, University of Gdansk, 63 Wita Stwosza, 80-308 Gdansk, Poland
Ewa Lojkowska: Laboratory of Plant Protection and Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk, 58 Abrahama, 80-307 Gdansk, Poland

IJERPH, 2021, vol. 18, issue 9, 1-15

Abstract: Pectinolytic bacteria from the genus Pectobacterium cause high economic losses in various crops, vegetables, and ornamentals including potato. Thus far, these strains have been isolated from distinct environments such as rotten or asymptomatic plants, soil, and waterways. The prevalence of soft rot Pectobacteriaceae in different depths of Pomeranian lakes was performed by a qualified scuba diver over 2 years of monitoring. It allowed for the isolation and broad characterization of a strain from the newly established species Pectobacterium aquaticum . Phylogenetic analysis on the sequences of dnaX and recA genes revealed the highest similarity of this strain to P. aquaticum CFBP 8637 T . In addition to the determination of analytical profile index (API 20E), we discovered that this strain possesses a smooth form of a lipopolysaccharide with O-polysaccharide consisting of mannose, glucose, and abequose. Moreover, the characterized strain, described as P. aquaticum IFB5637, produced plant-cell–wall-degrading enzymes, such as pectinases, cellulases, proteases, and was capable of macerating potato and chicory tissues under laboratory conditions. In view of more frequent irrigation of seed potato fields resulting from the ongoing climate warming, it is important to monitor the occurrence of potential disease-causing agents in natural waterways.

Keywords: Pectobacteriaceae; pectinolytic bacteria; soft rot; blackleg; monitoring; natural waterways (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2021
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.mdpi.com/1660-4601/18/9/5041/pdf (application/pdf)
https://www.mdpi.com/1660-4601/18/9/5041/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jijerp:v:18:y:2021:i:9:p:5041-:d:551727

Access Statistics for this article

IJERPH is currently edited by Ms. Jenna Liu

More articles in IJERPH from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().