Impact of Gingivitis on Circulating Neutrophil Reactivity and Gingival Crevicular Fluid Inflammatory Proteins

Helen M. Roberts, Zehra Yonel, Alpdogan Kantarci, Melissa M. Grant and Iain L. C. Chapple
Additional contact information
Helen M. Roberts: School of Dentistry, Institute of Clinical Science, University of Birmingham and Birmingham Community Healthcare Foundation Trust, 5 Mill Pool Way, Edgbaston, Birmingham B5 7ET, UK
Zehra Yonel: School of Dentistry, Institute of Clinical Science, University of Birmingham and Birmingham Community Healthcare Foundation Trust, 5 Mill Pool Way, Edgbaston, Birmingham B5 7ET, UK
Alpdogan Kantarci: Forsyth Institute, 245 First Street, Cambridge, MA 02142, USA
Melissa M. Grant: School of Dentistry, Institute of Clinical Science, University of Birmingham and Birmingham Community Healthcare Foundation Trust, 5 Mill Pool Way, Edgbaston, Birmingham B5 7ET, UK
Iain L. C. Chapple: School of Dentistry, Institute of Clinical Science, University of Birmingham and Birmingham Community Healthcare Foundation Trust, 5 Mill Pool Way, Edgbaston, Birmingham B5 7ET, UK

IJERPH, 2022, vol. 19, issue 10, 1-13

Abstract: Gingivitis is an extremely common oral inflammatory condition and can be induced in humans using an acute 21-day experimental gingivitis model. Neutrophils are known to be highly prevalent in the gingival crevice during gingival inflammation; however, the effect of gingivitis and the associated biofilm on peripheral blood neutrophils (PBN) is not well characterised. Thus, the aim of this study was to examine the impact of inflammation induced by experimental gingivitis and its resolution upon the function of PBN. Fifteen systemically healthy volunteers undertook a split-mouth 21-day experimental gingivitis study followed by a resolution phase of 14 days. PBN function, including reactive oxygen species (ROS) production, neutrophil extracellular trap (NET) release, directional chemotactic accuracy and expression of host mediators in gingival crevicular fluid (GCF), were measured at baseline (day 0), on day 21 and on day 35. NET formation and ROS production were significantly elevated at day 21. Chemotactic speed was also elevated in response to bacterial peptide fMLP at day 21. At day 35, ROS production in response to an Fcgamma stimulant, opsonised Staphylococcus aureus , remained elevated. The data presented suggest a lasting biological impact of the experimental gingivitis on PBN function even after clinical symptoms have abated.

Keywords: gingivitis; neutrophil; inflammation; gingival crevicular fluid; reactive oxygen species; neutrophil extracellular traps; cytokines; chemokines; chemotaxis (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2022
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.mdpi.com/1660-4601/19/10/6339/pdf (application/pdf)
https://www.mdpi.com/1660-4601/19/10/6339/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jijerp:v:19:y:2022:i:10:p:6339-:d:822049

Access Statistics for this article

IJERPH is currently edited by Ms. Jenna Liu

More articles in IJERPH from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().