Dispersion Stability of 14 Manufactured Nanomaterials for Ecotoxicity Tests Using Raphidocelis subcapitata

Seung-Hun Lee, Kiyoon Jung, Won Cheol Yoo, Jinwook Chung and Yong-Woo Lee
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Seung-Hun Lee: Department of Chemical and Molecular Engineering, Hanyang University, 55 Hanyangdaehakro, Sangrok-gu, Ansan 15588, Korea
Kiyoon Jung: Department of Chemical and Molecular Engineering, Hanyang University, 55 Hanyangdaehakro, Sangrok-gu, Ansan 15588, Korea
Won Cheol Yoo: Department of Chemical and Molecular Engineering, Hanyang University, 55 Hanyangdaehakro, Sangrok-gu, Ansan 15588, Korea
Jinwook Chung: R&D Center, Samsung Engineering Co., Ltd., 41 Maeyoung-ro, 269 Beon-gil, Youngtong-gu, Suwon 16523, Korea
Yong-Woo Lee: Department of Chemical and Molecular Engineering, Hanyang University, 55 Hanyangdaehakro, Sangrok-gu, Ansan 15588, Korea

IJERPH, 2022, vol. 19, issue 12, 1-13

Abstract: The development of nanotechnology has increased concerns about the exposure of ecosystems to manufactured nanomaterials, the toxicities of which are now being researched. However, when manufactured nanomaterials are mixed with algae in a culture medium for ecotoxicity tests, the results are vulnerable to distortion by an agglomeration phenomenon. Here, we describe a dispersion method commonly applicable to ecotoxicity tests for the 14 types of manufactured nanomaterials specified by the Organisation of Economic Co-operation and Development’s Sponsorship Programme, namely aluminum oxide (Al 2 O 3 ), carbon black, single-walled carbon nanotubes (SWCNTs), multi-walled carbon nanotubes (MWCNTs), cerium oxide (CeO 2 ), dendrimers, fullerene, gold (Au), iron (Fe), nanoclays, silver (Ag), silicon dioxide (SiO 2 ), titanium dioxide (TiO 2 ), and zinc oxide (ZnO). The type of dispersant, sonication time, and stirring speed were carefully considered. Consequently, 1500 mg/L of gum arabic was selected as a dispersant; for sonication time, 1 h was selected for dendrimers, 2 h for SiO 2 , 24 h for SWCNTs and Au, and 4 h for the other nanomaterials. Dispersion stability was achieved for all materials at a stirring speed of 200 rpm. To verify the effect of this dispersion method on ecotoxicity tests, toxicity was measured through cell counts for SWCNTs and TiO 2 using Raphidocelis subcapitata . The half-maximal effective concentrations (EC 50 ) were 18.0 ± 4.6 mg/L for SWCNTs and 316.6 ± 64.7 mg/L for TiO 2 .

Keywords: dispersion; ecotoxicity; manufactured nanomaterial; Raphidocelis subcapitata (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2022
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