Transcription Factor AhR, Cytokines IL-6 and IL-22 in Subjects with and without Peri-Implantitis: A Case Control-Study

Luis Ricardo Linard Martins, Kinga Grzech-Leśniak, Nidia Castro dos Santos, Lina J. Suárez, Gabriela Giro, Marta Ferreira Bastos and Jamil Awad Shibli
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Luis Ricardo Linard Martins: Department of Periodontology, Dental Research Division, Guarulhos University, Guarulhos 07023-070, Brazil
Kinga Grzech-Leśniak: Laser Laboratory at Dental Surgery Department, Medical University of Wroclaw, 50-425 Wroclaw, Poland
Nidia Castro dos Santos: Department of Periodontology, Dental Research Division, Guarulhos University, Guarulhos 07023-070, Brazil
Lina J. Suárez: Department of Periodontology, Dental Research Division, Guarulhos University, Guarulhos 07023-070, Brazil
Gabriela Giro: Department of Periodontology, Dental Research Division, Guarulhos University, Guarulhos 07023-070, Brazil
Marta Ferreira Bastos: Programa de Pós Graduação em Ciências do Envelhecimento, Universidade São Judas Tadeu, Rua Taquari, 546, Sao Paulo 03166-000, Brazil
Jamil Awad Shibli: Department of Periodontology, Dental Research Division, Guarulhos University, Guarulhos 07023-070, Brazil

IJERPH, 2022, vol. 19, issue 12, 1-10

Abstract: Peri-implantitis is a plaque-associated condition characterized by mucosal inflammation and subsequent progressive loss of supporting bone; it is caused by bacterial biofilm, but the host response triggered by bacterial stimulation promotes the release of cells and mediators that culminate in tissue destruction. The Aryl-hydrocarbon Receptor (AhR) is associated with IL-22 production by Th22 and Th17 CD4+ Th cells. The presence of IL-6 may promote the Th22 phenotype. The present case-control study evaluated the gene expression of AhR, IL-22, and IL-6 in the peri-implant tissues of healthy and peri-implantitis patients. Tissue biopsies were collected from thirty-five volunteers (15 healthy and 20 with peri-implantitis). A real-time PCR reaction was utilized to assess the AhR, IL-22, and IL-6 gene expression levels relative to the reference gene (GAPDH). The results were analyzed using the Mann–Whitney test with a significance level of 5%. Higher levels of gene expression of AhR and IL-6 were detected in peri-implantitis tissues. The IL-22 gene expression levels did not differ between groups. In conclusion, higher gene expression levels for AhR and IL-6 were detected in the soft tissues of peri-implantitis patients. IL-22 did not vary between conditions, which may indicate the loss of the immunomodulatory role of IL-22 in periimplantitis.

Keywords: gene expression; peri-implantitis; Th22 lymphocytes; transcription factor aryl-hydrocarbon receptor; interleukin-6; interleukin-22 (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2022
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