Profile of Bacterial Community and Antibiotic Resistance Genes in Typical Vegetable Greenhouse Soil

Xuexia Yuan, Yong Zhang, Chenxi Sun, Wenbo Wang, Yuanjuan Wu, Lixia Fan and Bing Liu
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Xuexia Yuan: Institute of Agricultural Standards and Testing Technology for Agri-Products, Shandong Academy of Agricultural Sciences & Shandong Provincial Key Laboratory of Test Technology on Food Quality and Safety, Jinan 250100, China
Yong Zhang: Shandong Provincial Land Surveying and Planning Institute, Jinan 250014, China
Chenxi Sun: Institute of Agricultural Standards and Testing Technology for Agri-Products, Shandong Academy of Agricultural Sciences & Shandong Provincial Key Laboratory of Test Technology on Food Quality and Safety, Jinan 250100, China
Wenbo Wang: Institute of Agricultural Standards and Testing Technology for Agri-Products, Shandong Academy of Agricultural Sciences & Shandong Provincial Key Laboratory of Test Technology on Food Quality and Safety, Jinan 250100, China
Yuanjuan Wu: Institute of Agricultural Standards and Testing Technology for Agri-Products, Shandong Academy of Agricultural Sciences & Shandong Provincial Key Laboratory of Test Technology on Food Quality and Safety, Jinan 250100, China
Lixia Fan: Institute of Agricultural Standards and Testing Technology for Agri-Products, Shandong Academy of Agricultural Sciences & Shandong Provincial Key Laboratory of Test Technology on Food Quality and Safety, Jinan 250100, China
Bing Liu: Resources and Environment Innovation Institute, School of Municipal and Environmental Engineering, Shandong Jianzhu University, Jinan 250101, China

IJERPH, 2022, vol. 19, issue 13, 1-15

Abstract: The use of vegetable greenhouse production systems has increased rapidly because of the increasing demand for food materials. The vegetable greenhouse production industry is confronted with serious environmental problems, due to their high agrochemical inputs and intensive utilization. Besides this, antibiotic-resistant bacteria, carrying antibiotic-resistance genes (ARGs), may enter into a vegetable greenhouse with the application of animal manure. Bacterial communities and ARGs were investigated in two typical vegetable-greenhouse-using counties with long histories of vegetable cultivation. The results showed that Proteobacteria , Firmicutes , Acidobacteria , Chloroflexi , and Gemmatimonadetes were the dominant phyla, while aadA , tetL , sul1 , and sul2 were the most common ARGs in greenhouse vegetable soil. Heatmap and principal coordinate analysis (PCoA) demonstrated that the differences between two counties were more significant than those among soils with different cultivation histories in the same county, suggesting that more effects on bacterial communities and ARGs were caused by soil type and manure type than by the accumulation of cultivation years. The positive correlation between the abundance of the intI gene with specific ARGs highlights the horizontal transfer potential of these ARGs. A total of 11 phyla were identified as the potential hosts of specific ARGs. Based on redundancy analysis (RDA), Ni and pH were the most potent factors determining the bacterial communities, and Cr was the top factor affecting the relative abundance of the ARGs. These results might be helpful in drawing more attention to the risk of manure recycling in the vegetable greenhouse, and further developing a strategy for practical manure application and sustainable production of vegetable greenhouses.

Keywords: antibiotic resistance genes; bacterial community; IntI gene; heavy metal; vegetable greenhouse (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2022
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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