Sub-Inhibitory Concentrations of Amoxicillin and Tylosin Affect the Biofilm Formation and Virulence of Streptococcus suis

Jing Zuo, Qingying Fan, Jinpeng Li, Baobao Liu, Bingqian Xue, Xiaoling Zhang, Li Yi and Yang Wang
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Jing Zuo: College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, China
Qingying Fan: College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, China
Jinpeng Li: College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, China
Baobao Liu: College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, China
Bingqian Xue: College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, China
Xiaoling Zhang: College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, China
Li Yi: Key Laboratory of Molecular Pathogen and Immunology of Animal of Luoyang, Luoyang 471000, China
Yang Wang: College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, China

IJERPH, 2022, vol. 19, issue 14, 1-12

Abstract: Streptococcus suis ( S. suis ) can form a protective biofilm during infection and lead to prolonged disease. Oral antibiotics are often used for treatment in clinical practice, but sub-inhibitory concentration levels often exist due to low oral absorption rate, resulting in disease deterioration. The purpose of this study was to investigate the effects of Amoxicillin and Tylosin on the biofilm formation and virulence of S. suis HA9801 at sub-inhibitory concentration. We first determined that the test groups (1/4MIC Amoxicillin and Tylosin) could significantly increase the amount of biofilm formation without affecting bacterial growth. The LD50 value of the test groups was significantly higher than that of the control group in the mouse infection model. In the mouse infection model, the LD50 value of the experimental group was significantly increased, but the tissue bacterial load was significantly decreased. Further RT-PCR analysis showed that the expression levels of virulence-related genes in the experimental group were significantly reduced. Our study suggests that both Amoxicillin and Tylosin at sub-inhibitory concentrations could enhance the biofilm formation ability of S. suis HA9801 and reduce its virulence to form persistent infection.

Keywords: antibiotics; biofilm; Streptococcus suis; sub-inhibitory concentration; virulence (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2022
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