Cannabidiol (CBD) Protects Adipose-Derived Mesenchymal Stem Cells (ASCs) against Endoplasmic Reticulum Stress Development and Its Complications

Anna Kowalczuk (), Krzysztof Marycz, Katarzyna Kornicka-Garbowska, Justyna Kornicka, Magdalena Bujalska-Zadrożny and Sylwia Groborz
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Anna Kowalczuk: National Medicines Institute, 00-725 Warsaw, Poland
Krzysztof Marycz: International Institute of Translational Medicine, 55-114 Wisznia Mała, Poland
Katarzyna Kornicka-Garbowska: International Institute of Translational Medicine, 55-114 Wisznia Mała, Poland
Justyna Kornicka: Faculty of Electronics, Photonics and Microsystems, Wrocław University of Science and Technology, 50-372 Wrocław, Poland
Magdalena Bujalska-Zadrożny: Department of Pharmacodynamics, Centre for Preclinical, Research and Technology (CePT), Medical University of Warsaw, 02-097 Warsaw, Poland
Sylwia Groborz: International Institute of Translational Medicine, 55-114 Wisznia Mała, Poland

IJERPH, 2022, vol. 19, issue 17, 1-25

Abstract: Background: Recent studies suggested that individuals with metabolic disorders have altered function of adipocytes and adipose stem cell subpopulations, which impairs tissue homeostasis, promoting insulin resistance and diabetes development. The non-psychoactive phytocannabinoid CBD was found to modulate adipose tissue metabolism, however, its exact role in controlling ASCs’ fate is still poorly understood. Objectives: This investigation aimed to elucidate whether pretreatment of ASCs with CBD can protect against ER stress development and maintain the cytophysiological properties of cells. Methods: Human ASCs were cultured under control and adipogenic conditions. Prior to the experiments, cells in the experimental group were pretreated with CBD following the addition of an ER stress inducer—tunicamycin. After the experiments, the cells were subsequently tested for expression of the apoptotic, ER stress, and anti-inflammatory-related genes using RT-qPCR. Oxidative stress was analysed with flow cytometric assays. Results: Cells pretreated with CBD displayed decreased apoptosis and enhanced proliferation rate. Additionally, the expression of pro-inflammatory cytokines and miRNAs was significantly reduced. The obtained results also demonstrated an obvious reduction in intracellular accumulated ROS and NO, as well as mitigated ER stress through the down-regulation of IRE-1 , PERK , CHOP , and ATF6 transcripts upon CBD treatment. Conclusion: The presented data provide the evidence that CBD protects ASCs against ER stress development and its complications and, thus, offers new insights for the management of obesity through the regulation of adipose tissue dynamics.

Keywords: adipogenesis; adipose stem cells; CBD; ER stress; apoptosis; oxidative stress (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2022
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