Immobilization of EreB on Acid-Modified Palygorskite for Highly Efficient Degradation of Erythromycin

Shensheng Ni, Chunyu Li, Yicheng Yu, Dongze Niu, Jie Zhu, Dongmin Yin, Chongqing Wang, Wenfan Zhang, Xingmei Jiang and Jianjun Ren ()
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Shensheng Ni: Institute of Urban and Rural Mining, Changzhou University, No. 21 Gehu Road, Wujin District, Changzhou 213164, China
Chunyu Li: Institute of Urban and Rural Mining, Changzhou University, No. 21 Gehu Road, Wujin District, Changzhou 213164, China
Yicheng Yu: Jiangsu Key Laboratory of Phylogenomics and Comparative Genomics, School of Life Sciences, Jiangsu Normal University, No. 101 Shanghai Road, Tongshan District, Xuzhou 221116, China
Dongze Niu: Institute of Urban and Rural Mining, Changzhou University, No. 21 Gehu Road, Wujin District, Changzhou 213164, China
Jie Zhu: National-Local Joint Engineering Research Center for Biomass Refining and High-Quality Utilization, Changzhou University, No. 21 Gehu Road, Wujin District, Changzhou 213164, China
Dongmin Yin: Institute of Urban and Rural Mining, Changzhou University, No. 21 Gehu Road, Wujin District, Changzhou 213164, China
Chongqing Wang: Beijing General Station of Animal Husbandry, No. 21 Chaoqian Road, Changping District, Beijing 100101, China
Wenfan Zhang: Institute of Urban and Rural Mining, Changzhou University, No. 21 Gehu Road, Wujin District, Changzhou 213164, China
Xingmei Jiang: Bijie Institute of Animal Husbandry and Veterinary Sciences, De Gou Ma Jia Yuan, Qixingguan District, Bijie 551700, China
Jianjun Ren: Institute of Urban and Rural Mining, Changzhou University, No. 21 Gehu Road, Wujin District, Changzhou 213164, China

IJERPH, 2022, vol. 19, issue 17, 1-12

Abstract: Erythromycin is one of the most commonly used macrolide antibiotics. However, its pollution of the ecosystem is a significant risk to human health worldwide. Currently, there are no effective and environmentally friendly methods to resolve this issue. Although erythromycin esterase B (EreB) specifically degrades erythromycin, its non-recyclability and fragility limit the large-scale application of this enzyme. In this work, palygorskite was selected as a carrier for enzyme immobilization. The enzyme was attached to palygorskite via a crosslinking reaction to construct an effective erythromycin-degradation material (i.e., EreB@modified palygorskite), which was characterized using FT-IR, SEM, XRD, and Brunauer–Emmett–Teller techniques. The results suggested the successful modification of the material and the loading of the enzyme. The immobilized enzyme had a higher stability over varying temperatures (25–65 °C) and pH values (6.5–10.0) than the free enzyme, and the maximum rate of reaction (V max ) and the turnover number (k cat ) of the enzyme increased to 0.01 mM min −1 and 169 min −1 , respectively, according to the enzyme-kinetics measurements. The EreB@modified palygorskite maintained about 45% of its activity after 10 cycles, and degraded erythromycin in polluted water to 20 mg L −1 within 300 min. These results indicate that EreB could serve as an effective immobilizing carrier for erythromycin degradation at the industrial scale.

Keywords: EreB; erythromycin degradation; immobilization; palygorskite (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2022
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