Characterization and Degradation Pathways of Microbacterium resistens MZT7, A Novel 17 β -Estradiol-Degrading Bacterium

Peng Hao, Sicheng Wu, Xiqing Zhang, Changlong Gou, Yuqiong Wang, Lixia Wang, Yanbin Zhu, Wangdui Basang and Yunhang Gao ()
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Peng Hao: College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China
Sicheng Wu: College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China
Xiqing Zhang: College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China
Changlong Gou: College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao 028000, China
Yuqiong Wang: College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao 028000, China
Lixia Wang: Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China
Yanbin Zhu: Institute of Animal Husbandry and Veterinary Medicine, Tibet Academy of Agriculture and Animal Husbandry Science, Lhasa 850009, China
Wangdui Basang: Institute of Animal Husbandry and Veterinary Medicine, Tibet Academy of Agriculture and Animal Husbandry Science, Lhasa 850009, China
Yunhang Gao: College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China

IJERPH, 2022, vol. 19, issue 17, 1-14

Abstract: Due to the ecotoxicity of 17β-estradiol (E2), residual E2 in the environment poses potential risks to human and animal health and ecosystems. Biodegradation is considered one of the most effective strategies to remove E2 from the environment. Here, a novel, efficient E2-degrading bacterial strain Microbacterium resistens MZT7 was isolated from activated sludge and characterized. The genome of strain MZT7 contained 4,011,347 bp nucleotides with 71.26% G + C content and 3785 coding genes. There was 86.7% transformation efficiency of 10 mg/L E2 by strain MZT7 after incubation for 5 d at optimal temperature (30 °C) and pH (7.0). This strain was highly tolerant to ranges in pH (5.0–11.0), temperature (20–40 °C), and salinity (2–8%). Adding sources of carbon (glucose, maltose, sucrose, or lactose) or nitrogen sources (urea, peptone, or beef extract) promoted the degradation of E2 by strain MZT7. However, when yeast extract was added as a nitrogen source, the degradation efficiency of E2 was inhibited. Metabolites were analyzed by LC-MS and three metabolic pathways of E2 degradation were proposed. Further, the intermediates dehydroepiandrosterone and androsta-1,4-diene-3,17-dione were detected, as well as identification of kshB and fadD3 genes by KEGG, confirming one E2 degradation pathway. This study provided some insights into E2 biodegradation.

Keywords: 17 ? -estradiol; Microbacterium resistens MZT7; biodegradation; metabolic pathway; genome analyses (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2022
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