Liposomes Containing Amaranth Unsaponifiable Matter and Soybean Lunasin Suppress ROS Production in Fibroblasts and Reduced Interleukin Production in Macrophages

Gloria Dávila-Ortiz (), Erick Damian Castañeda-Reyes, Carlos Ignacio Juárez-Palomo, María de Jesús Perea-Flores, Ricardo Pérez-Pastén-Borja, Yazmín Karina Márquez-Flores and Elvira González de Mejía
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Gloria Dávila-Ortiz: Unidad Profesional Adolfo López Mateos, Departamento de Ingeniería Bioquímica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Campus Zacatenco, Madero, Ciudad de México 07738, Mexico
Erick Damian Castañeda-Reyes: Unidad Profesional Adolfo López Mateos, Departamento de Ingeniería Bioquímica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Campus Zacatenco, Madero, Ciudad de México 07738, Mexico
Carlos Ignacio Juárez-Palomo: Unidad Profesional Adolfo López Mateos, Departamento de Ingeniería Bioquímica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Campus Zacatenco, Madero, Ciudad de México 07738, Mexico
María de Jesús Perea-Flores: Unidad Profesional Adolfo López Mateos, Centro de Nanociencias y Micro y Nanotecnologías, Instituto Politécnico Nacional (IPN), Av. Luis Enrique Erro s/n, Zacatenco, Alcaldía Gustavo A. Madero, Ciudad de México 07738, Mexico
Ricardo Pérez-Pastén-Borja: Unidad Profesional Adolfo López Mateos, Departamento de Farmacia, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Campus Zacatenco, Madero, Ciudad de México 07738, Mexico
Yazmín Karina Márquez-Flores: Unidad Profesional Adolfo López Mateos, Departamento de Farmacia, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Campus Zacatenco, Madero, Ciudad de México 07738, Mexico
Elvira González de Mejía: Department of Food Science and Human Nutrition, University of Illinois, Urbana-Champaign, IL 61801, USA

IJERPH, 2022, vol. 19, issue 18, 1-11

Abstract: Inflammation is a normal response in defense to agents that may cause damage to the human body. When inflammation becomes chronic, reactive oxygen species (ROS) are produced; which could lead to diseases such as cancer. The aim was to assess liposomes’ antioxidant and anti-inflammatory capacity loaded with amaranth unsaponifiable matter and soybean lunasin (UM + LunLip) in an in vitro model using fibroblasts and macrophages. To evaluate ROS production, fibroblasts CHON-002 ABAP were added to promote ROS production; and the cells were treated with UM + LunLip. For inflammation markers production, lipopolysaccharides (LPS)-stimulated RAW 264.7 and peritoneal macrophages were treated with empty liposomes (EmLip), liposomes loaded with unsaponifiable matter (UMLip), liposomes loaded with lunasin (LunLip), and UM + LunLip. ROS production was significantly decreased by 77% ( p < 0.05) when fibroblasts were treated with UM + LunLip at 2 mg lunasin/mL compared with the control treated with ABAP. Treatment with UMLip was the most effective in reducing tumor necrosis factor-α (71–90%) and interleukin-6 (43–55%, p < 0.001). Both liposomes containing unsaponifiable matter (UMLip and UM + LunLip) were more effective than EmLip or LunLip. In conclusion, amaranth unsaponifiable matter-loaded liposomes are effective in decreasing pro-inflammatory cytokine production.

Keywords: liposomes; interleukin-6; tumor necrosis factor-?; ROS-production; amaranth squalene; soybean lunasin; inflammation (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2022
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