Quantification of Plasma 8-Isoprostane by High-Performance Liquid Chromatography with Tandem Mass Spectrometry in a Case-Control Study of Lung Cancer

Lin Ma, Dongxiao Sun, Guangli Xiu, Philip Lazarus, Anil Vachani, Trevor M. Penning, Alexander S. Whitehead and Joshua E. Muscat ()
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Lin Ma: State Environmental Protection Key Lab of Environmental Risk Assessment and Control on Chemical Processes, School of Resources & Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China
Dongxiao Sun: Department of Pharmacology, Mass Spectrometry Core Facility, The Pennsylvania State College of Medicine, Hershey, PA 17033, USA
Guangli Xiu: State Environmental Protection Key Lab of Environmental Risk Assessment and Control on Chemical Processes, School of Resources & Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China
Philip Lazarus: Department of Pharmaceutical Sciences, College of Pharmacy and Pharmaceutical Sciences, Washington State University, Spokane, WA 99210, USA
Anil Vachani: Department of Medicine, Pulmonary, Allergy, and Critical Care Division, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
Trevor M. Penning: Department of Systems Pharmacology & Translational Therapeutics, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
Alexander S. Whitehead: Department of Pharmacology, Center for Pharmacogenetics, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
Joshua E. Muscat: Department of Public Health Sciences, Pennsylvania State College of Medicine, Hershey, PA 17033, USA

IJERPH, 2022, vol. 19, issue 19, 1-13

Abstract: Aim: 8-iso-prostaglandin F2α is a biomarker of lipid peroxidation, and one of the most commonly used measures of oxidative stress. It is an established biomarker of lung cancer risk. It is commonly measured by enzyme-linked immunosorbent assay. Given its importance, we developed a stable isotope dilution UPLC-tandem mass spectrometric method for the rapid determination of 8-isoprostane in blood. Methods: We tested the discriminatory capability of the method in 49 lung cancer patients, 55 benign lung nodule patients detected by chest X-ray, and 41 patients with chronic obstructive pulmonary disease (COPD) or asthma. Results: Significant differences were found in mean 8-isoprostane levels between the three groups ( p = 0.027), and post-hoc tests found higher levels in the lung cancer patients than in patients with benign nodules ( p = 0.032) and COPD/asthma ( p = 0.014). The receiving operating characteristic area under the curve (AUC) was 0.69 for differentiating the lung cancer group from the benign nodule group, and 0.7 for differentiating from the COPD/asthma group. Conclusions: The UPLC-MS/MS-based method is an efficient analytical tool for measuring 8-isoprostane plasma concentrations. The results suggest exploring its utility as a marker for early lung cancer screening.

Keywords: oxidative stress; biomarkers; lung cancer; isoprostanes (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2022
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