Inhibition of LPS-Induced Microglial Activation by the Ethyl Acetate Extract of Pueraria mirifica

Nattinee Jantaratnotai, Anusorn Thampithak, Pongsak Utaisincharoen, Darawan Pinthong and Pimtip Sanvarinda ()
Additional contact information
Nattinee Jantaratnotai: Department of Pharmacology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
Anusorn Thampithak: Division of Pharmacology and Biopharmaceutical Sciences, Faculty of Pharmaceutical Sciences, Burapha University, Chonburi 20131, Thailand
Pongsak Utaisincharoen: Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
Darawan Pinthong: Department of Pharmacology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
Pimtip Sanvarinda: Department of Pharmacology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand

IJERPH, 2022, vol. 19, issue 19, 1-11

Abstract: Microglial activation has been found to play a crucial role in various neurological disorders. Proinflammatory substances overproduced by activated microglia, such as cytokines, chemokines, reactive oxygen species, and nitric oxide (NO), can result in neuroinflammation that further exacerbates the course of the diseases. This study aimed to explore the anti-inflammatory effect of the ethyl acetate extract of Pueraria mirifica on microglial activation. Lipopolysaccharide (LPS)-induced inflammation was used as a model to investigate the effects of P. mirifica on HAPI (highly aggressive proliferating immortalized), a rat microglial cell line. Administration of ethyl acetate extract from the tuberous roots of P. mirifica to HAPI cells dose-dependently reduced NO production and iNOS expression induced by LPS. Attenuation of IRF-1 (interferon regulatory factor-1) induction, one of the transcription factors governing iNOS expression, suggested that the inhibitory effect on NO production by the plant extract was at least partially mediated through this transcription factor. In addition, LPS-stimulated mRNA expression of MCP-1 (monocyte chemoattractant protein-1), IL-6 (interleukin-6), and TNF-α (tumor necrosis factor-α) was also suppressed with P. mirifica extract pretreatment. This study indicates that the ethyl acetate extract of P. mirifica could potentially serve as an anti-inflammatory mediator and may be useful in relieving the severity of neurological diseases where microglia play a role.

Keywords: Pueraria mirifica; microglia; inflammation; nitric oxide; cytokines (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2022
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.mdpi.com/1660-4601/19/19/12920/pdf (application/pdf)
https://www.mdpi.com/1660-4601/19/19/12920/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jijerp:v:19:y:2022:i:19:p:12920-:d:937147

Access Statistics for this article

IJERPH is currently edited by Ms. Jenna Liu

More articles in IJERPH from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().