Differential Gene Expression and Metabolic Pathway Analysis of Cladophora rupestris under Pb Stress Conditions

Lei Liu, Lusheng Zhang, Lingyun Zhao, Qiuyu Chen, Qian Zhang, Deju Cao () and Zhaowen Liu ()
Additional contact information
Lei Liu: Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China
Lusheng Zhang: Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China
Lingyun Zhao: Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China
Qiuyu Chen: Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China
Qian Zhang: Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China
Deju Cao: Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China
Zhaowen Liu: Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China

IJERPH, 2022, vol. 19, issue 21, 1-14

Abstract: This study aimed to analyze the transcriptome of C. rupestris under Pb 2+ stress by using high-throughput sequencing technology, observe the changes of gene expression and metabolic pathway after three and five days under 1.0 and 5.0 mg/L of Pb 2+ treatment, and analyze the differentially expressed genes (DEGs) and related functional genes after Pb 2+ treatment. Metabolic pathways were revealed through Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Results show that DEGs increased significantly with the increase of Pb 2+ concentration and stress time. A total of 32 genes were closely related to Pb 2+ stress response. GO analysis identified two major transporter proteins, namely, ATP-binding transport protein-related (ABC transporters) and zinc finger CCHC domain containing protein (Zfp) in C. rupestris . Pthr19248, pthr19211, Zfp pthr23002, Zfp p48znf pthr12681, Zfp 294 pthr12389, and Zfp pthr23067 played important roles against Pb 2+ toxicity and its absorption in C. rupestris . KEGG pathway analysis suggested that ABCA1, ATM, and ABCD3 were closely related to Pb 2+ absorption. Pb 2+ stress was mainly involved in metallothionein (MT), plant hormone signal transduction, ABC transporters, and glutathione (GSH) metabolism.

Keywords: C. rupestris; Pb; transcriptome; DEGs; transporter proteins; MT; GSH (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2022
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.mdpi.com/1660-4601/19/21/13910/pdf (application/pdf)
https://www.mdpi.com/1660-4601/19/21/13910/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jijerp:v:19:y:2022:i:21:p:13910-:d:953570

Access Statistics for this article

IJERPH is currently edited by Ms. Jenna Liu

More articles in IJERPH from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().