Keratinolytic Properties of Aspergillus clavatus Promising for Biodegradation

Svetlana Timorshina (), Elizaveta Popova, Valeriana Kreyer, Nina Baranova and Alexander Osmolovskiy
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Svetlana Timorshina: Department of Microbiology, Faculty of Biology, Lomonosov Moscow State University, 119991 Moscow, Russia
Elizaveta Popova: Department of Microbiology, Faculty of Biology, Lomonosov Moscow State University, 119991 Moscow, Russia
Valeriana Kreyer: Department of Microbiology, Faculty of Biology, Lomonosov Moscow State University, 119991 Moscow, Russia
Nina Baranova: Department of Microbiology, Faculty of Biology, Lomonosov Moscow State University, 119991 Moscow, Russia
Alexander Osmolovskiy: Department of Microbiology, Faculty of Biology, Lomonosov Moscow State University, 119991 Moscow, Russia

IJERPH, 2022, vol. 19, issue 21, 1-17

Abstract: The high demand for keratinolytic enzymes and the modest presentation of fungal keratinase diversity studies in scientific sources cause a significant interest in identifying new fungal strains of keratinase producers, isolating new enzymes and studying their properties. Four out of the 32 cultures showed a promising target activity on protein-containing agar plates— Aspergillus amstelodami A6, A. clavatus VKPM F-1593, A. ochraceus 247, and Cladosporium sphaerospermum 1779. The highest values of keratinolytic activity were demonstrated by extracellular proteins synthesized by Aspergillus clavatus VKPM F-1593 cultivated under submerged conditions on a medium containing milled chicken feathers. The enzyme complex preparation was obtained by protein precipitation from the culture liquid with ammonium sulfate, subsequent dialysis, and lyophilization. The fraction of a pure enzyme with keratinolytic activity (pI 9.3) was isolated by separating the extracellular proteins of A. clavatus VKPM F-1593 via isoelectric focusing. The studied keratinase was an alkaline subtilisin-like non-glycosylated protease active over a wide pH range with optimum keratinolysis at pH 8 and 50 °C.

Keywords: Aspergillus clavatus; fungal proteases; micromycetes; keratinases; keratin waste; biodegradation (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2022
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