Extraction, Purification, Characterization and Application in Livestock Wastewater of S Sulfur Convertase
Xintian Li,
Wei Zhai,
Xinran Duan,
Changlong Gou,
Min Li,
Lixia Wang,
Wangdui Basang,
Yanbin Zhu and
Yunhang Gao ()
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Xintian Li: College of Animal Science and Technology, Jilin Agricultural University, Changchun 130000, China
Wei Zhai: College of Animal Science and Technology, Jilin Agricultural University, Changchun 130000, China
Xinran Duan: College of Animal Science and Technology, Jilin Agricultural University, Changchun 130000, China
Changlong Gou: College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao 028000, China
Min Li: College of Animal Science and Technology, Jilin Agricultural University, Changchun 130000, China
Lixia Wang: Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China
Wangdui Basang: Institute of Animal Husbandry and Veterinary Medicine, Tibet Academy of Agricultural and Animal Husbandry Science, Lhasa 850009, China
Yanbin Zhu: Institute of Animal Husbandry and Veterinary Medicine, Tibet Academy of Agricultural and Animal Husbandry Science, Lhasa 850009, China
Yunhang Gao: College of Animal Science and Technology, Jilin Agricultural University, Changchun 130000, China
IJERPH, 2022, vol. 19, issue 23, 1-14
Abstract:
Sulfide is a toxic pollutant in the farming environment. Microbial removal of sulfide always faces various biochemical challenges, and the application of enzymes for agricultural environmental remediation has promising prospects. In this study, a strain of Cellulosimicrobium sp. was isolated: numbered strain L1. Strain L1 can transform S 2− , extracellular enzymes play a major role in this process. Next, the extracellular enzyme was purified, and the molecular weight of the purified sulfur convertase was about 70 kDa. The sulfur convertase is an oxidase with thermal and storage stability, and the inhibitor and organic solvent have little effect on its activity. In livestock wastewater, the sulfur convertase can completely remove S 2− . In summary, this study developed a sulfur convertase and provides a basis for the application in environmental remediation.
Keywords: sulfide; extracellular enzyme; purification; enzymatic property; livestock wastewater (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2022
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