Evaluation Study of xMAP TIER Assay on a Microsphere-Based Platform for Detecting First-Line Anti-Tuberculosis Drug Resistance

Xichao Ou, Zhiguo Zhang, Bing Zhao, Zexuan Song, Shengfen Wang, Wencong He, Shaojun Pei, Dongxin Liu, Ruida Xing, Hui Xia and Yanlin Zhao ()
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Xichao Ou: National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Zhiguo Zhang: Tuberculosis Dispensary of Changping District, Beijing 102202, China
Bing Zhao: National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Zexuan Song: National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Shengfen Wang: National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Wencong He: National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Shaojun Pei: School of Public Health, Peking University, Beijing 100191, China
Dongxin Liu: National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Ruida Xing: National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Hui Xia: National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Yanlin Zhao: National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China

IJERPH, 2022, vol. 19, issue 24, 1-9

Abstract: Early diagnosis of drug susceptibility for tuberculosis (TB) patients could guide the timely initiation of effective treatment. We evaluated a novel multiplex xMAP TIER (Tuberculosis-Isoniazid-Ethambutol-Rifampicin) assay based on the Luminex xMAP system to detect first-line anti-tuberculous drug resistance. Deoxyribonucleic acid samples from 353 Mycobacterium tuberculosis clinical isolates were amplified by multiplex polymerase chain reaction, followed by hybridization and analysis through the xMAP system. Compared with the broth microdilution method, the sensitivity and specificity of the xMAP TIER assay for detecting resistance was 94.9% (95%CI, 90.0–99.8%) and 98.9% (95%CI, 97.7–100.0%) for rifampicin; 89.1% (95%CI, 83.9–94.3%) and 100.0% (95%CI, 100.0–100.0%) for isoniazid; 82.1% (95% CI, 68.0–96.3%) and 99.7% (95% CI, 99.0–100.0%) for ethambutol. With DNA sequencing as the reference standard, the sensitivity and specificity of xMAP TIER for detecting resistance were 95.0% (95% CI, 90.2–99.8%) and 99.6% (95% CI, 98.9–100.0%) for rifampicin; 96.9% (95% CI, 93.8–99.9%) and 100.0% (95% CI, 100.0–100.0%) for isoniazid; 86.1% (95% CI, 74.8–97.4%) and 100.0% (95% CI, 100.0–100.0%) for ethambutol. The results achieved showed that the xMAP TIER assay had good performance for detecting first-line anti-tuberculosis drug resistance, and it has the potential to diagnose drug-resistant tuberculosis more accurately due to the addition of more optimal design primers and probes on open architecture xMAP system.

Keywords: Mycobacteirum tuberculosis; diagnosis; xMAP; drug resistance (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2022
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