Enhanced Biodegradation of Phenylurea Herbicides by Ochrobactrum anthrophi CD3 Assessment of Its Feasibility in Diuron-Contaminated Soils

Lara-Moreno Alba, Morillo Esmeralda and Villaverde Jaime
Additional contact information
Lara-Moreno Alba: Department of Agrochemistry, Environmental Microbiology and Soil Conservation, Institute of Natural Resources and Agrobiology of Seville, Spanish National Research Council (IRNAS-CSIC), 41012 Seville, Spain
Morillo Esmeralda: Department of Agrochemistry, Environmental Microbiology and Soil Conservation, Institute of Natural Resources and Agrobiology of Seville, Spanish National Research Council (IRNAS-CSIC), 41012 Seville, Spain
Villaverde Jaime: Department of Agrochemistry, Environmental Microbiology and Soil Conservation, Institute of Natural Resources and Agrobiology of Seville, Spanish National Research Council (IRNAS-CSIC), 41012 Seville, Spain

IJERPH, 2022, vol. 19, issue 3, 1-17

Abstract: The phenylurea herbicides are persistent in soil and water, making necessary the de-velopment of techniques for their removal from the environment. To identify new options in this regard, bacterial strains were isolated from a soil historically managed with pesticides. Ochrobactrum anthropi CD3 showed the ability to remove completely herbicides such as diuron, linuron, chlorotoluron and fluometuron from aqueous solution, and up to 89% of isoproturon. In the case of diuron and linuron, their main metabolite, 3,4-dichloroaniline (3,4-DCA), which has a higher toxicity than the parent compounds, was formed, but remained in solution without further degradation. O. anthropi CD3 was also tested for bioremediation of two different agricultural soils artificially contaminated with diuron, employing bioremediation techniques: (i) biostimulation, using a nutrient solution (NS), (ii) bioaugmentation, using O. anthropi CD3, and iii) bioavailability enhancement using 2-hydroxypropyl-β-cyclodextrin (HPBCD). When bioaugmentation and HPBCD were jointly applied, 50% of the diuron initially added to the soil was biodegraded in a range from 4.7 to 0.7 d. Also, 3,4-DCA was degraded in soil after the strain was inoculated. At the end of the soil biodegradation assay an ecotoxicity test confirmed that after inoculating O. anthropi CD3 the toxicity was drastically reduced.

Keywords: phenylurea herbicides; 3,4-dichloroaniline; soil; bioremediation; bioaugmentation; biostimulation; 2-hydroxypropyl-β-cyclodextrin (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2022
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.mdpi.com/1660-4601/19/3/1365/pdf (application/pdf)
https://www.mdpi.com/1660-4601/19/3/1365/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jijerp:v:19:y:2022:i:3:p:1365-:d:734405

Access Statistics for this article

IJERPH is currently edited by Ms. Jenna Liu

More articles in IJERPH from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().