In Vitro Biocompatibility of Several Children’s Toothpastes on Human Gingival Fibroblasts

María Pilar Pecci-Lloret, Sergio López-García, Francisco Javier Rodríguez-Lozano, Pablo Álvarez-Novoa and David García-Bernal
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María Pilar Pecci-Lloret: Gerodontology and Special Care Dentistry Unit, Morales Meseguer Hospital, Faculty of Medicine, University of Murcia, 30100 Murcia, Spain
Sergio López-García: Department of Stomatology, Faculty of Medicine and Dentistry, Universitat de Valencia, 46010 Valencia, Spain
Francisco Javier Rodríguez-Lozano: Gerodontology and Special Care Dentistry Unit, Morales Meseguer Hospital, Faculty of Medicine, University of Murcia, 30100 Murcia, Spain
Pablo Álvarez-Novoa: Department of Surgery and Medical-Surgical Specialties, School of Medicine and Dentistry, Universidade de Santiago de Compostela, 15705 La Coruña, Spain
David García-Bernal: Cellular Therapy and Hematopoietic Transplant Group, Instituto Murciano de Investigación Biosanitaria Virgen de la Arrixaca, University of Murcia, 30120 Murcia, Spain

IJERPH, 2022, vol. 19, issue 5, 1-11

Abstract: The European Academy of Paediatric Dentistry has recommended fluoride toothpastes from the eruption of the first teeth in children. Toothpastes stay in the mouth in contact with human gingival fibroblasts (hGFs) for a long time. Thus, the objective of this study was to compare the cytotoxicity of five different commonly used children’s toothpastes: Oral B Kids +3 (Procter & Gamble, Alicante, Spain), Fluor Kin Calcium (Kin, Madrid, Spain), PHB Junior (PHB, Barcelona, Spain), Colgate +3 (Colgate Palmolive, Madrid, Spain) and Vitis Kids (Dentaid, Valencia, Spain) on hGFs. The children’s toothpastes were exposed to hGFs at different concentrations (1:1, 1:2, 1:4). Afterwards, several tests were performed: MTT assays, cell cycle analyses, cell cytoskeleton staining assays, apoptosis/necrosis assays, and ICP-MS and ion chromatography. Oral B displayed the lowest cytotoxicity and was the toothpaste with the highest fluoride ion release; meanwhile, the other toothpastes were cytotoxic (*** p < 0.0001); Fluor Kin being the one with the lowest fluoride ion release. Among all the toothpastes analyzed, Oral B exhibited the best results in vitro in terms of biocompatibility. Future evaluations, both in vitro and in vivo, are required to confirm the biocompatibility of sodium lauryl sarcosinate and sodium lauryl sulfate containing toothpastes.

Keywords: children’s toothpastes; biocompatibility; SLS; fluoride (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2022
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