Comparison between Single and Multi-LED Emitters for Photodynamic Therapy: An In Vitro Study on Enterococcus faecalis and Human Gingival Fibroblasts

Morena Petrini, Tania Vanessa Pierfelice, Emira D’Amico, Teocrito Carlesi, Giovanna Iezzi, Camillo D’Arcangelo, Silvia Di Lodovico, Adriano Piattelli and Simonetta D’Ercole
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Morena Petrini: Department of Medical, Oral and Biotechnological Sciences, University G. d’Annunzio of Chieti-Pescara, Via dei Vestini 31, 66013 Chieti, Italy
Tania Vanessa Pierfelice: Department of Medical, Oral and Biotechnological Sciences, University G. d’Annunzio of Chieti-Pescara, Via dei Vestini 31, 66013 Chieti, Italy
Emira D’Amico: Department of Medical, Oral and Biotechnological Sciences, University G. d’Annunzio of Chieti-Pescara, Via dei Vestini 31, 66013 Chieti, Italy
Teocrito Carlesi: Department of Medical, Oral and Biotechnological Sciences, University G. d’Annunzio of Chieti-Pescara, Via dei Vestini 31, 66013 Chieti, Italy
Giovanna Iezzi: Department of Medical, Oral and Biotechnological Sciences, University G. d’Annunzio of Chieti-Pescara, Via dei Vestini 31, 66013 Chieti, Italy
Camillo D’Arcangelo: Department of Medical, Oral and Biotechnological Sciences, University G. d’Annunzio of Chieti-Pescara, Via dei Vestini 31, 66013 Chieti, Italy
Silvia Di Lodovico: Department of Pharmacy, University “G. d’Annunzio”, Chieti-Pescara, Via dei Vestini, 31, 66100 Chieti, Italy
Adriano Piattelli: School of Dentistry, Saint Camillus International University of Health and Medical Sciences, Via di Sant’Alessandro 8, 00131 Rome, Italy
Simonetta D’Ercole: Department of Medical, Oral and Biotechnological Sciences, University G. d’Annunzio of Chieti-Pescara, Via dei Vestini 31, 66013 Chieti, Italy

IJERPH, 2022, vol. 19, issue 5, 1-10

Abstract: Aim of the study: The aim was to evaluate the effects of two LED devices, TL-01 and TL-03 in photodynamic therapy (PDT), on Enterococcus faecalis and on human gingival fibroblasts (HGFs). TL-01, characterized by a single emitter, irradiates one periodontal site at a time, whereas the multi-led device (TL-03) irradiates all vestibular sites of a single arch at a time. Methods: E. faecalis bacterial suspensions and HGFs were incubated for 45 min with Aladent gel (ALAD) containing 5-aminolevulinic acid and then exposed to LED devices (ALAD-PDT), having different distance and timing of irradiation (TL-01 N (0.5 mm, for 7 min), TL-03 N (0.5 mm, 15 min) and TL-03 F (30.0 mm, 15 min)). For bacterial suspension, the colony forming units and the live/dead staining were evaluated after 24 h, while the protoporphyrin IX (PpIX) content was monitored in all phases of the experimentation. For HGFs, the cell viability, proliferation, cell morphology, and adhesion were evaluated at 24 h. Results: Both TL-01 and TL-03 showed a significant reduction of bacterial load. The photoinactivation was inversely proportional to the PpIX accumulation. TL-01 and TL-03 promoted proliferation and adhesion of HGFs. Conclusions: Both tested devices for ALAD-PDT were equally effective in significantly reducing Enterococcus faecalis growth and in promoting HGFs proliferation and adhesion, in vitro.

Keywords: photodynamic therapy; LED devices; Enterococcus faecalis; human gingival fibroblast (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2022
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