UV-C Light-Based Surface Disinfection: Analysis of Its Virucidal Efficacy Using a Bacteriophage Model

Stefan A. Rudhart, Frank Günther, Laura Dapper, Boris A. Stuck and Stephan Hoch
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Stefan A. Rudhart: Department of Otolaryngology, Head and Neck Surgery, University Hospital Marburg, Philipps-University Marburg, 35043 Marburg, Germany
Frank Günther: Department of Medical Microbiology and Hygiene, University Hospital Marburg, Philipps-University Marburg, 35043 Marburg, Germany
Laura Dapper: Department of Medical Microbiology and Hygiene, University Hospital Marburg, Philipps-University Marburg, 35043 Marburg, Germany
Boris A. Stuck: Department of Otolaryngology, Head and Neck Surgery, University Hospital Marburg, Philipps-University Marburg, 35043 Marburg, Germany
Stephan Hoch: Department of Otolaryngology, Head and Neck Surgery, University Hospital Marburg, Philipps-University Marburg, 35043 Marburg, Germany

IJERPH, 2022, vol. 19, issue 6, 1-9

Abstract: Background: The reprocessing of medical devices has become more complex due to increasing hygiene requirements. Previous studies showed satisfactory bactericidal disinfection effects of UV-C light in rigid and flexible endoscopes. Especially in the context of the current COVID-19 pandemic, virucidal properties are of high importance. In the present study, the virucidal efficacy of UV-C light surface disinfection was analyzed. Methods: MS-2 bacteriophages were applied to the test samples and irradiated by UV-C light using the UV Smart D25 device; unirradiated test samples were used as controls. A dilution series of the samples was mixed with 1 × 10 8 Escherichia coli and assayed. Results: 8.6 × 10 12 pfu could be harvested from the unprocessed test samples. In the control group without UV-C exposure, a remaining contamination of 1.2 × 10 12 pfu was detected, resulting in a procedural baseline reduction rate with a LOG 10 reduction factor of 0.72. The LOG 10 reduction factor was found to be 3.0 after 25 s of UV-C light exposure. After 50 and 75 s of UV-C radiation LOG 10 reduction factors 4.2 and 5.9, respectively, were found, with all reductions being statistically significantly different to baseline. Conclusions: The tested UV system seems to provide a significant virucidal effect after a relatively short irradiation time.

Keywords: endoscopes; otorhinolaryngology; COVID-19; MS-2; UV light; D25; disinfection; surface disinfection; virus; adenovirus (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2022
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