Characterisation of Salmonella Enteritidis ST11 and ST1925 Associated with Human Intestinal and Extra-Intestinal Infections in Singapore

Kyaw Thu Aung, Wei Ching Khor, Kar Hui Ong, Wei Ling Tan, Zhi Ning Wong, Jia Quan Oh, Wai Kwan Wong, Brian Zi Yan Tan, Matthias Maiwald, Nancy Wen Sim Tee, Timothy Barkham, Tse Hsien Koh, Anders Dalsgaard, Swaine L. Chen, Joergen Schlundt and Lee Ching Ng
Additional contact information
Kyaw Thu Aung: National Centre for Food Science, Singapore Food Agency, Singapore 718837, Singapore
Wei Ching Khor: National Centre for Food Science, Singapore Food Agency, Singapore 718837, Singapore
Kar Hui Ong: National Centre for Food Science, Singapore Food Agency, Singapore 718837, Singapore
Wei Ling Tan: National Centre for Food Science, Singapore Food Agency, Singapore 718837, Singapore
Zhi Ning Wong: National Centre for Food Science, Singapore Food Agency, Singapore 718837, Singapore
Jia Quan Oh: National Centre for Food Science, Singapore Food Agency, Singapore 718837, Singapore
Wai Kwan Wong: Animal & Veterinary Service, National Parks Board, Singapore 718827, Singapore
Brian Zi Yan Tan: Animal & Veterinary Service, National Parks Board, Singapore 718827, Singapore
Matthias Maiwald: Department of Pathology and Laboratory Medicine, KK Women’s and Children’s Hospital, Singapore 229899, Singapore
Nancy Wen Sim Tee: Department of Laboratory Medicine, National University Hospital, Singapore 119074, Singapore
Timothy Barkham: Department of Laboratory Medicine, Tan Tock Seng Hospital, Singapore 308433, Singapore
Tse Hsien Koh: Department of Pathology, Singapore General Hospital, Singapore 169856, Singapore
Anders Dalsgaard: Nanyang Technological University Food Technology Centre (NAFTEC), Singapore 637459, Singapore
Swaine L. Chen: NUHS Infectious Diseases Translational Research Programme and Department of Medicine, Division of Infectious Diseases, Yong Loo Lin School of Medicine, National University of Singapore, 1E Kent Ridge Road, NUHS Tower Block, Singapore 119228, Singapore
Joergen Schlundt: Nanyang Technological University Food Technology Centre (NAFTEC), Singapore 637459, Singapore
Lee Ching Ng: Environmental Health Institute, National Environment Agency, Singapore 138667, Singapore

IJERPH, 2022, vol. 19, issue 9, 1-14

Abstract: Salmonella Enteritidis is a major foodborne pathogen worldwide. In this study, a total of 276 S. enteritidis isolates, collected between 2016 and 2017 from human, food and farm/slaughterhouse samples, were studied to enhance the understanding of the epidemiology of human salmonellosis in Singapore. Results showed all 276 isolates belonged either to ST1925 (70.3%) or ST11 (29.7%), with ST11 being significantly more frequent in extra-intestinal isolates and chicken isolates. Food isolates, most of which were from poultry, showed the highest prevalence of resistance (33–37%) against beta-lactams or beta-lactams/beta-lactamase inhibitor combination (ampicillin, piperacillin and ampicillin/sulbactam). The analysis showed the detection of genes associated with resistance to aminoglycoside genes (99.6%), tetracycline (55.1%), and beta-lactams (14.9%) of all isolates. Nine types of plasmids were found in 266 isolates; the most common incompatibility group profiles were IncFIB(S)-IncFII(S)-IncX1 (72.2%) and IncFIB(S)-IncFII(S) (15.8%). Most plasmid harbouring isolates from chicken (63.6%, 14/22) and from human (73.8%, 175/237) shared the same plasmid profile (IncFIB(S)-IncFII(S)-IncX1). SNP analysis showed clustering of several isolates from poultry food products and human isolates, suggesting phylogenetic relatedness among these isolates. Lastly, this study provides important epidemiological insights on the application of phenotypic and next-generation sequencing (NGS) tools for improved food safety and public health surveillance and outbreak investigation of S. enteritidis .

Keywords: Salmonella Enteritidis; sequence type; salmonellosis; epidemiology; antimicrobial resistance; human; food; farm environment; integrated surveillance (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2022
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