The Mechanism of miR-21-5p/TSP-1-Mediating Exercise on the Function of Endothelial Progenitor Cells in Aged Rats

Xiaoke Chen, Kejia Xie, Xinzheng Sun, Chengzhu Zhang and Hui He ()
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Xiaoke Chen: School of Sports Science, Beijing Sport University, Beijing 100084, China
Kejia Xie: School of Sports Science, Beijing Sport University, Beijing 100084, China
Xinzheng Sun: School of Sports Science, Beijing Sport University, Beijing 100084, China
Chengzhu Zhang: School of Sports Science, Beijing Sport University, Beijing 100084, China
Hui He: China Institute of Sport and Health Science, Beijing Sport University, Beijing 100084, China

IJERPH, 2023, vol. 20, issue 2, 1-13

Abstract: (1) Background: The declined function of peripheral circulating endothelial progenitor cells (EPCs) in aging individuals resulted in decreased endothelial cell regeneration and vascular endothelial function. Improving EPCs function in aging individuals plays an important role in preventing cardiovascular diseases. (2) Methods: Thirty aged (18-month-old) male Sprague-Dawley rats were randomly divided into control and exercise groups. An aerobic exercise intervention was performed 5 days/week for 8 weeks. EPCs functions, miR-21-5p, and TSP-1 expressions were detected after the intervention. The senescence rate, proliferation, and migration of EPCs were examined after overexpression of miR-21-5p and inhibition of TSP-1 expression. (3) Results: The senescence rate, proliferation, and migration of EPCs in exercise groups were significantly improved after exercise intervention. The miR-21-5p expression was increased and the TSP-1 mRNA expression was decreased in the EPCs after the intervention. miR-21-5p overexpression can improve EPCs function and inhibit TSP-1 expression but has no effect on senescence rate. Inhibition of TSP-1 expression could improve the function and reduce the senescence rate. (4) Conclusions: Our results indicate that long-term aerobic exercise can improve the functions of EPCs in aging individuals by downregulating TSP-1 expression via miR-21-5p, which reveals the mechanism of exercise in improving cardiovascular function.

Keywords: aerobic exercise; endothelial progenitor cells; miR-21-5p; thrombospondin 1 (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2023
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