Antimicrobial Effects of Non-Thermal Atmospheric Pressure Plasma on Oral Microcosm Biofilms

Jiyeon Lee, Sungbo Cho and Hee-Eun Kim ()
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Jiyeon Lee: Department of Health Science, Gachon University Graduate School of Public Health, Incheon 21936, Republic of Korea
Sungbo Cho: Department of Electronic Engineering, Gachon University, Seongnam-si 13120, Republic of Korea
Hee-Eun Kim: Department of Health Science, Gachon University Graduate School of Public Health, Incheon 21936, Republic of Korea

IJERPH, 2023, vol. 20, issue 3, 1-10

Abstract: We comparatively evaluated the antibacterial effects of non-thermal atmospheric pressure plasma (NTAPP) on oral microcosm biofilms. Oral microcosm biofilms, which are derived from inoculation with human saliva, were cultured on 48 hydroxyapatite disks for 6 days. The prepared biofilms were divided into three different daily treatment groups: distilled water for 1 min, 0.12% chlorhexidine (CHX) for 1 min, and NTAPP for 5 min. Using a quantitative light-induced fluorescence-digital camera, the red fluorescence intensity of the biofilms was measured as red/green ratios (Ratio R/G ) before and after treatment. Total and aciduric bacteria were counted as colony-forming units. Using live/dead bacterial staining, bacterial viability was calculated as the Ratio G/G+R . Ratio R/G was approximately 0.91-fold lower in the NTAPP group than in the CHX group on day 1 of treatment ( p = 0.001), and approximately 0.94-fold lower on both days 2 and 3 ( p < 0.001). The number of total bacteria was higher in the NTAPP group than in the CHX group, but not significantly different. The number of aciduric bacteria was lowest in the CHX group ( p < 0.001). However, bacterial viability was lowest in the NTAPP group. Restricted bacterial aggregation was observed in the NTAPP group. These findings suggest that NTAPP may more effectively reduce the pathogenicity of oral microcosm biofilms than 0.12% CHX.

Keywords: antimicrobial effect; atmospheric pressure plasma; biofilm; non-thermal plasma; oral disease; plasma (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2023
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