Degradation Characteristics and Remediation Ability of Contaminated Soils by Using β -HCH Degrading Bacteria

Qing Chen, Huijun Shi, Yanpeng Liang (), Litang Qin, Honghu Zeng () and Xiaohong Song
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Qing Chen: College of Environmental Science and Engineering, Guilin University of Technology, Guilin 541004, China
Huijun Shi: College of Environmental Science and Engineering, Guilin University of Technology, Guilin 541004, China
Yanpeng Liang: Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, Guilin 541004, China
Litang Qin: College of Environmental Science and Engineering, Guilin University of Technology, Guilin 541004, China
Honghu Zeng: College of Environmental Science and Engineering, Guilin University of Technology, Guilin 541004, China
Xiaohong Song: College of Environmental Science and Engineering, Guilin University of Technology, Guilin 541004, China

IJERPH, 2023, vol. 20, issue 4, 1-17

Abstract: Three degradation strains that can utilize β -Hexachlorocyclohexanes ( β -HCH) as the sole carbon source were isolated from the soil substrate of constructed wetland under long-term β -HCH stress, and they were named A1, J1, and M1. Strains A1 and M1 were identified as Ochrobactrum sp. and strain J1 was identified as Microbacterium oxydans sp. by 16S rRNA gene sequence analysis. The optimum conditions for degradation with these three strains, A1, J1, and M1, were pH = 7, 30 °C, and 5% inoculum amount, and the degradation rates of 50 μg/L β -HCH under these conditions were 58.33%, 51.96%, and 50.28%, respectively. Degradation characteristics experiments showed that root exudates could increase the degradation effects of A1 and M1 on β -HCH by 6.95% and 5.82%, respectively. In addition, the degradation bacteria A1 and J1 mixed in a ratio of 1:1 had the highest degradation rate of β -HCH, which was 69.57%. An experiment on simulated soil remediation showed that the compound bacteria AJ had the best effect on promoting the degradation of β -HCH in soil within 98 d, and the degradation rate of β -HCH in soil without root exudates was 60.22%, whereas it reached 75.02% in the presence of root exudates. The addition of degradation bacteria or degradation bacteria-root exudates during soil remediation led to dramatic changes in the community structure of the soil microorganisms, as well as a significant increase in the proportion of aerobic and Gram-negative bacterial groups. This study can enrich the resources of β -HCH degrading strains and provided a theoretical basis for the on-site engineering treatment of β -HCH contamination.

Keywords: ? -HCH; microbial degradation; soil remediation; microbial community (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2023
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