Damage Effect of Amorphous Carbon Black Nanoparticle Aggregates on Model Phospholipid Membranes: Surface Charge, Exposure Concentration and Time Dependence

Xiao-Feng Wang, Kun Xu, Xin-Rui Li, Ya-Xin Liu and Jie-Min Cheng ()
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Xiao-Feng Wang: College of Geography and Environment, Shandong Normal University, Jinan 250358, China
Kun Xu: College of Geography and Environment, Shandong Normal University, Jinan 250358, China
Xin-Rui Li: College of Geography and Environment, Shandong Normal University, Jinan 250358, China
Ya-Xin Liu: College of Geography and Environment, Shandong Normal University, Jinan 250358, China
Jie-Min Cheng: College of Geography and Environment, Shandong Normal University, Jinan 250358, China

IJERPH, 2023, vol. 20, issue 4, 1-12

Abstract: Commercial nano-scale carbon blacks (CB) are being harnessed widely and may impose potentially hazardous effects because of their unique properties, especially if they have been modified to grow reactive functional groups on their surface. Cytotoxicity of CB has been well studied but the membrane damage mechanisms and role of surface modification are still open to debate. Negatively and positively charged giant unilamellar vesicles (GUVs) were prepared using three lipids as model cell membranes to examine the mechanistic damage of CB and MCB (modified by acidic potassium permanganate) aggregates. Optical images showed that both anionic CB and MCB disrupted the positively charged but not the negatively charged GUVs. This disruption deteriorated with the rise and extension of exposure concentration and time. Lipids extraction caused by CBNs (CB and MCB together are called CBNs) was found. MCB caused more severe disruption than CB. MCB was enveloped into vesicles through an endocytosis-like process at 120 mg/L. MCB mediated the gelation of GUVs, perhaps through C-O-P bonding bridges. The lower hydrodynamic diameter and more negative charges may have been responsible for the distinction effect of MCB over CB. The adhesion and bonding of CBNs to the membrane were favored by electrostatic interaction and the practical application of CBNs warrants more attention.

Keywords: model membrane damage; modified carbon black nanoparticle; concentration dependent; electrostatic interaction; giant unilamellar vesicles (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2023
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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