Endometrial Injury Upregulates Expression of Receptivity Genes in Women with Implantation Failure

Onder Celik, Arzu Yurci, Aynur Ersahin, Nur D. Gungor, Nilufer Celik, Mustafa D. Ozcil, Serdar Dogan, Semih Dalkilic, Lutfiye Dalkilic, Ulun Ulug, Sudenaz Celik and Andrea Tinelli ()
Additional contact information
Onder Celik: Private Clinic Obstetrics and Gynecology, 64000 Usak, Turkey
Arzu Yurci: In Vitro Fertilization (IVF), Andrology and Genetics Center, Memorial Bahcelievler Hospital, 34180 Istanbul, Turkey
Aynur Ersahin: Department of Obstetrics and Gynecology, Bahcesehir University Goztepe Medicalpark Hospital, 34732 Istanbul, Turkey
Nur D. Gungor: Department of Obstetrics and Gynecology, Bahcesehir University Goztepe Medicalpark Hospital, 34732 Istanbul, Turkey
Nilufer Celik: Department of Biochemistry, Behcet Uz Children’s Hospital, 35210 Izmir, Turkey
Mustafa D. Ozcil: Department of Obstetrics and Gynecology, Faculty of Medicine, Mustafa Kemal University, 31060 Hatay, Turkey
Serdar Dogan: Department of Medical Biochemistry, Faculty of Medicine, Mustafa Kemal University, 31060 Hatay, Turkey
Semih Dalkilic: Molecular Biology and Genetics Program, Department of Biology, Faculty of Science, Firat University, 23000 Elazig, Turkey
Lutfiye Dalkilic: Molecular Biology and Genetics Program, Department of Biology, Faculty of Science, Firat University, 23000 Elazig, Turkey
Ulun Ulug: Department of Obstetrics and Gynecology, School of Medicine, Halic University, 34060 Istanbul, Turkey
Sudenaz Celik: Medical Faculty, Sofia University “St. Kliment Ohridski”, 1407 Sofia, Bulgaria
Andrea Tinelli: Department of Obstetrics and Gynecology, CERICSAL (Centro di RIcerca Clinica SALentino), “Veris Delli Ponti Hospital”, 73020 Lecce, Italy

IJERPH, 2023, vol. 20, issue 5, 1-14

Abstract: Background: Homeobox genes A10 (HOXA10) and A11 (HOXA11), members of the abdominal B gene family, are responsible for embryonic survival and implantation. This study was planned to investigate whether endometrial injury alters the expression of both transcripts in women with implantation failure. Methods: A total of 54 women with implantation failure were divided into two equal groups as experimental (scratching) and sham (no scratching). Participants in the scratching group were exposed to endometrial injury in the mid-luteal phase, and those in the sham group were exposed to endometrial flushing. The scratching group, but not the sham group, underwent prior endometrial sampling. A second endometrial sampling was performed on the scratching group in the mid-luteal phase of the following cycle. The mRNA and protein levels of the HOXA10 and 11 transcripts were determined in endometrial samples collected before and after injury/flushing. Participants in each group underwent IVF/ET in the cycle after the second endometrial sampling. Results: Endometrial injury caused a 60.1-fold ( p < 0.01) increase in HOXA10 mRNA and a 9.0-fold increase in HOXA11 mRNA ( p < 0.02). Injury resulted in a significant increase in both HOXA10 ( p < 0.001) and HOXA11 protein expression ( p < 0.003). There was no significant change in HOXA10 and 11 mRNA expressions after flushing. Clinical pregnancy, live birth, and miscarriage rates of the both groups were similar. Conclusions: Endometrial injury increases homeobox transcript expression at both mRNA and protein levels.

Keywords: implantation failure; endometrium; homeobox gene; mRNA; protein; fertility outcome (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2023
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