Three Experimental Common High-Risk Procedures: Emission Characteristics Identification and Source Intensity Estimation in Biosafety Laboratory

Zhijian Liu (), Jiabin Lv, Zheng Zhang, Juntao Ma, Yangfan Song, Minnan Wu, Guoqing Cao and Jiacheng Guo
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Zhijian Liu: Department of Power Engineering, North China Electric Power University, Baoding 071003, China
Jiabin Lv: Department of Power Engineering, North China Electric Power University, Baoding 071003, China
Zheng Zhang: Department of Power Engineering, North China Electric Power University, Baoding 071003, China
Juntao Ma: Department of Power Engineering, North China Electric Power University, Baoding 071003, China
Yangfan Song: Department of Power Engineering, North China Electric Power University, Baoding 071003, China
Minnan Wu: Department of Power Engineering, North China Electric Power University, Baoding 071003, China
Guoqing Cao: Institute of Building Environment and Energy, China Academy of Building Research, Beijing 100013, China
Jiacheng Guo: Department of Power Engineering, North China Electric Power University, Baoding 071003, China

IJERPH, 2023, vol. 20, issue 5, 1-11

Abstract: Biosafety laboratory is an important place to study high-risk microbes. In biosafety laboratories, with the outbreak of infectious diseases such as COVID-19, experimental activities have become increasingly frequent, and the risk of exposure to bioaerosols has increased. To explore the exposure risk of biosafety laboratories, the intensity and emission characteristics of laboratory risk factors were investigated. In this study, high-risk microbe samples were substituted with Serratia marcescens as the model bacteria. The resulting concentration and particle size segregation of the bioaerosol produced by three experimental procedures (spill, injection, and sample drop) were monitored, and the emission sources’ intensity were quantitatively analyzed. The results showed that the aerosol concentration produced by injection and sample drop was 10 3 CFU/m 3 , and that by sample spill was 10 2 CFU/m 3 . The particle size of bioaerosol is mainly segregated in the range of 3.3–4.7 μ m. There are significant differences in the influence of risk factors on source intensity. The intensity of sample spill, injection, and sample drop source is 3.6 CFU/s, 78.2 CFU/s, and 664 CFU/s. This study could provide suggestions for risk assessment of experimental operation procedures and experimental personnel protection.

Keywords: bioaerosol; risk assessment; gaussian mixture model; quantitative analysis (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2023
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