Leonotis nepetifolia Transformed Root Extract Reduces Pro-Inflammatory Cytokines and Promotes Tissue Repair In Vitro

Przemysław Sitarek, Tomasz Kowalczyk, Tomasz Śliwiński, Sophia Hatziantoniou, Nikolitsa Soulintzi, Rafal Pawliczak and Joanna Wieczfinska ()
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Przemysław Sitarek: Department of Biology and Pharmaceutical Botany, Medical University of Lodz, Muszynskiego 1, 90-151 Lodz, Poland
Tomasz Kowalczyk: Department of Molecular Biotechnology and Genetics, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland
Tomasz Śliwiński: Laboratory of Medical Genetics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland
Sophia Hatziantoniou: Laboratory of Pharmaceutical Technology, Department of Pharmacy, School of Health Sciences, University of Patras, 26504 Patras, Greece
Nikolitsa Soulintzi: Laboratory of Pharmaceutical Technology, Department of Pharmacy, School of Health Sciences, University of Patras, 26504 Patras, Greece
Rafal Pawliczak: Department of Immunopathology, Medical University of Lodz, Zeligowskiego 7/9, Bldg 2, Rm 177, 90-752 Lodz, Poland
Joanna Wieczfinska: Department of Immunopathology, Medical University of Lodz, Zeligowskiego 7/9, Bldg 2, Rm 177, 90-752 Lodz, Poland

IJERPH, 2023, vol. 20, issue 6, 1-12

Abstract: Inflammation is closely related to asthma and its defining feature: airway remodeling. The aim of this study was to determine the effects of extracts of normal (NR) and transformed (TR) Leonotis nepetifolia roots on respiratory cells and against the gingival epithelium. Extracts from NR and TR roots were added to lung fibroblast, bronchial epithelial and gingival fibroblast cell lines, in the presence of HRV-16 infection, to determine their impact on inflammation. The expression of inflammatory cytokines (IL-6, IL-1β, GM-CSF and MCAF) as well as total thiol contents were assessed. The TR extract inhibited rhinovirus-induced IL-6 and IL-1β expression in all tested airway cells ( p < 0.05). Additionally, the extract decreased GM-CSF expression in bronchial epithelial cells. The tested extracts had positive effects on total thiol content in all tested cell lines. The TR root extract demonstrated wound healing potential. While both tested extracts exhibited anti-inflammatory and antioxidative effects, they were stronger for the TR extract, possibly due to higher concentrations of beneficial metabolites such as phenols and flavonoids. Additionally, wound healing activity was demonstrated for the TR root extract. These results suggest that TR root extract may become a promising therapeutic agent in the future.

Keywords: Leonotis nepetifolia root extract; transformed root extract; inflammation; cytokines; airway cells (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2023
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