Sensitivity of Mouse Lung Nuclear Receptors to Electronic Cigarette Aerosols and Influence of Sex Differences: A Pilot Study

Shikha Sharma, Dustin Rousselle, Erik Parker, Carolyn Damilola Ekpruke, Rachel Alford, Maksat Babayev, Sarah Commodore and Patricia Silveyra ()
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Shikha Sharma: Department of Environmental and Occupational Health, School of Public Health, Indiana University, Bloomington, IN 47405, USA
Dustin Rousselle: Department of Environmental and Occupational Health, School of Public Health, Indiana University, Bloomington, IN 47405, USA
Erik Parker: Biostatistics Consulting Center, Department of Epidemiology and Biostatistics, School of Public Health, Indiana University, Bloomington, IN 47405, USA
Carolyn Damilola Ekpruke: Department of Environmental and Occupational Health, School of Public Health, Indiana University, Bloomington, IN 47405, USA
Rachel Alford: Department of Environmental and Occupational Health, School of Public Health, Indiana University, Bloomington, IN 47405, USA
Maksat Babayev: Department of Environmental and Occupational Health, School of Public Health, Indiana University, Bloomington, IN 47405, USA
Sarah Commodore: Department of Environmental and Occupational Health, School of Public Health, Indiana University, Bloomington, IN 47405, USA
Patricia Silveyra: Department of Environmental and Occupational Health, School of Public Health, Indiana University, Bloomington, IN 47405, USA

IJERPH, 2024, vol. 21, issue 6, 1-17

Abstract: The emerging concern about chemicals in electronic cigarettes, even those without nicotine, demands the development of advanced criteria for their exposure and risk assessment. This study aims to highlight the sensitivity of lung nuclear receptors (NRs) to electronic cigarette e-liquids, independent of nicotine presence, and the influence of the sex variable on these effects. Adult male and female C57BL/6J mice were exposed to electronic cigarettes with 0%, 3%, and 6% nicotine daily (70 mL, 3.3 s, 1 puff per min/30 min) for 14 days, using the inExpose full body chamber (SCIREQ). Following exposure, lung tissues were harvested, and RNA extracted. The expression of 84 NRs was determined using the RT 2 profiler mRNA array (Qiagen). Results exhibit a high sensitivity to e-liquid exposure irrespective of the presence of nicotine, with differential expression of NRs, including one (females) and twenty-four (males) in 0% nicotine groups compared to non-exposed control mice. However, nicotine-dependent results were also significant with seven NRs (females), fifty-three NRs (males) in 3% and twenty-three NRs (female) twenty-nine NRs (male) in 6% nicotine groups, compared to 0% nicotine mice. Sex-specific changes were significant, but sex-related differences were not observed. The study provides a strong rationale for further investigation.

Keywords: electronic cigarette; E-liquids; nuclear receptors; nicotine; sex differences; lungs; chemicals of concern in ENDS (Electronic Nicotine Delivery Systems) (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2024
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