Diesel Exhaust Particles Induce the Over expression of Tumor Necrosis Factor-? (TNF-?) Gene in Alveolar Macrophages and Failed to Induce Apoptosis through Activation of Nuclear Factor-?B (NF-?B)

Ramzi M. Kafoury and Michael C. Madden
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Ramzi M. Kafoury: Center of Environmental Health and Molecular Toxicology Laboratory, Jackson State University, Jackson, Mississippi, USA
Michael C. Madden: Human Exposure Section, United States Environmental Protection Agency, Chapel Hill, North Carolina

IJERPH, 2005, vol. 2, issue 1, 1-7

Abstract: Exposure to particulate matter (PM 2.5-10 ), including diesel exhaust particles (DEP) has been reported to induce lung injury and exacerbation of asthma and chronic obstructive pulmonary disease. Alveolar macrophages play a major role in the lung’s response to inhaled particles and therefore, are a primary target for PM 2.5-10 effect. The molecular and cellular events underlying DEP-induced toxicity in the lung, however, remain unclear. To determine the effect of DEP on alveolar macrophages, RAW 264.7 cells were grown in RPMI 1640 with supplements until confluency. RAW 264.7 cultures were exposed to Hank’s buffered saline solution (vehicle), vehicle containing an NF-?B inhibitor, BAY11-7082 (25?M with 11/2 hr pre-incubation), or vehicle containing DEP (250?g/ml) in the presence or absence of BAY11-7082 (25?M with 11/2 hr pre-incubation) for 4 hr and TNF-? release was determined by enzyme-linked immunosorbent assay and confirmed by western blots. RAW 264.7 apoptotic response was determined by DNA fragmentation assays. U937 cells treated with campothecin (4 ?g/ml x 3 hr), an apoptosis-inducing agent, were used as positive control. We report that exposure to the carbonaceous core of DEP induces significant release of TNF-? in a concentration-dependent fashion (31 ± 4 pg/ml, n = 4, p = 0.08; 162 ± 23 pg/ml, n = 4, p

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JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2005
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