Photoirradiation of Retinyl Palmitate in Ethanol with Ultraviolet Light - Formation of Photodecomposition Products, Reactive Oxygen Species, and Lipid Peroxides

Qingsu Xia, Jun J. Yin, Wayne G. Wamer, Shu-Hui Cherng, Mary D. Boudreau, Paul C. Howard, Hongtao Yu and Peter P. Fu
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Qingsu Xia: National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USA
Jun J. Yin: Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, College Park, MD 20740, USA
Wayne G. Wamer: Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, College Park, MD 20740, USA
Shu-Hui Cherng: National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USA
Mary D. Boudreau: National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USA
Paul C. Howard: National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USA
Hongtao Yu: Department of Chemistry, Jackson State University, Jackson, MS 39217, USA
Peter P. Fu: National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USA

IJERPH, 2006, vol. 3, issue 2, 1-6

Abstract: We have previously reported that photoirradiation of retinyl palmitate (RP), a storage and ester form of vitamin A (retinol), with UVA light resulted in the formation of photodecomposition products, generation of reactive oxygen species, and induction of lipid peroxidation. In this paper, we report our results following the photoirradiation of RP in ethanol by an UV lamp with approximately equal UVA and UVB light. The photodecomposition products were separated by reversed-phase HPLC and characterized spectroscopically by comparison with authentic standards. The identified products include: 4-keto-RP, 11-ethoxy-12-hydroxy-RP, 13-ethoxy-14-hydroxy-RP, anhydroretinol (AR), and trans - and cis -15-ethoxy-AR. Photoirradiation of RP in the presence of a lipid, methyl linoleate, resulted in induction of lipid peroxidation. Lipid peroxidation was inhibited when sodium azide was present during photoirradiation which suggests free radicals were formed. Our results demonstrate that, similar to irradiation with UVA light, RP can act as a photosensitizer leading to free radical formation and induction of lipid peroxidation following irradiation with UVB light.

Keywords: Retinyl palmitate; UVB; Photoirradiation; Lipid peroxidation (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2006
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