Induction of Cell Death, DNA Strand Breaks, and Cell Cycle Arrest in DU145 Human Prostate Carcinoma Cell Line by Benzo[a]pyrene and Benzo[a]pyrene-7,8-diol-9,10-epoxide

Onyinye Nwagbara, Selina F. Darling-Reed, Alicia Tucker, Cynthia Harris, Michael Abazinge, Ronald D. Thomas and Richard D. Gragg
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Onyinye Nwagbara: Environmental Sciences Institute, Florida A&M University, Tallahassee, FL 32307, USA
Selina F. Darling-Reed: College of Pharmacy and Pharmaceutical Sciences, Florida A&M University, Tallahassee, FL 32307, USA
Alicia Tucker: College of Pharmacy and Pharmaceutical Sciences, Florida A&M University, Tallahassee, FL 32307, USA
Cynthia Harris: Institute of Public Health, Florida A&M University, Tallahassee, FL 32307, USA
Michael Abazinge: Environmental Sciences Institute, Florida A&M University, Tallahassee, FL 32307, USA
Ronald D. Thomas: College of Pharmacy and Pharmaceutical Sciences, Florida A&M University, Tallahassee, FL 32307, USA
Richard D. Gragg: Environmental Sciences Institute, Florida A&M University, Tallahassee, FL 32307, USA

IJERPH, 2007, vol. 4, issue 1, 1-5

Abstract: Benzo[a]pyrene (B[a]P), a polycyclic aromatic hydrocarbon, is a major environmental pollutant. In this study, the effects of this carcinogen/mutagen and one of its metabolites, benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE), on human prostate carcinoma cell line DU145, were examined. Cell viability, DNA damage, and cell cycle progression were evaluated as toxic end-points. We have shown that B[a]P and BPDE inhibited cell viability following 48 hr of exposure. Furthermore, comet assay analyses revealed that both B[a]P and BPDE induced DNA strand breaks in a concentration -dependent fashion. Flow cytometric analyses showed that about 70 % of DU145 cells were arrested by B[a]P at the G1 phase, while about 76% were arrested at G1 phase by BPDE. These data reveal that B[a]P and BPDE are cytotoxic and genotoxic to DU145 prostate cancer cells.

Keywords: Benzo[a]pyrene; benzo[a]pyrene-7; 8-diol-9; 10-epoxide; DU145; cell viability; DNA damage; cell cycle (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2007
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