Genotoxicity of Air Borne Particulates Assessed by Comet and the Salmonella Mutagenicity Test in Jeddah, Saudi Arabia

Sufian M. ElAssouli, Mohamed H. AlQahtani and Waleed Milaat
Additional contact information
Sufian M. ElAssouli: Medical Biology Department, 2Family & Community Medicine Department, College of Medicine, King Abdulaziz University, P. O. Box 80205, Jeddah 21589, Saudi Arabia
Mohamed H. AlQahtani: Medical Biology Department, 2Family & Community Medicine Department, College of Medicine, King Abdulaziz University, P. O. Box 80205, Jeddah 21589, Saudi Arabia
Waleed Milaat: Medical Biology Department, 2Family & Community Medicine Department, College of Medicine, King Abdulaziz University, P. O. Box 80205, Jeddah 21589, Saudi Arabia

IJERPH, 2007, vol. 4, issue 3, 1-8

Abstract: Fine airborne respirable particulates less than 10 micrometer (PM10) are considered one of the top environmental public health concerns, since they contain polycyclic aromatic hydrocarbons (PAHs) which are among the major carcinogenic compounds found in urban air. The objective of this study is to assess the genotoxicity of the ambient PM10 collected at 11 urban sites in Jeddah, Saudi Arabia. The PM10 extractable organic matter (EOM) was examined for its genotoxicity by the single cell gel electrophoresis (SCGE) comet assay and the Salmonella mutagenicity (Ames) test .Gas chromatography-mass spectrometry was used to quantify 16 PAH compounds in four sites. Samples from oil refinery and heavy diesel vehicles traffic sites showed significant DNA damage causing comet in 20-44% of the cells with tail moments ranging from 0.5-2.0 compared to samples from petrol driven cars and residential area, with comet in less than 2% of the cells and tail moments of 3 , mean while residential and reference sites caused 2-15 rev /m 3 . The genotoxicity of the EOM in both tests directly correlated with the amount of organic particulate and the PAHs concentrations in the air samples. The PAHs concentrations ranged between 0.83 ng/m 3 in industrial and heavy diesel vehicles traffic sites to 0.18 ng /m 3 in the residential area. Benzo(ghi)pyrene was the major PAH components and at one site it represented 65.4 % of the total PAHs. Samples of the oil refinery site were more genotoxic in the SCGE assay than samples from the heavy diesel vehicles traffic site, despite the fact that both sites contain almost similar amount of PAHs. The opposite was true for the mutagenicity in the Ames test. This could be due to the nature of the EOM in both sites. These findings confirm the genotoxic potency of the PM10 organic extracts to which urban populations are exposed.

Keywords: Genotoxicity; PM10 air particulats; comet; salmonella mutagenicity; PAHs (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2007
References: View complete reference list from CitEc
Citations: View citations in EconPapers (2)

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